4X41

Crystal Structure of Protein Arginine Methyltransferase PRMT8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-22
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 2 2 21
Unit cell lengths	68.159, 78.236, 203.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.727 - 3.500
R-factor	0.2339
Rwork	0.231
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.019
Data reduction software	SCALA
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1819)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	3.620
High resolution limit [Å]	3.500	7.420	3.500
Rmerge	0.196	0.064	0.478
Rmeas	0.239	0.078	0.586
Rpim	0.133	0.043	0.332
Total number of observations	38801
Number of reflections	13561
<I/σ(I)>	5.7
Completeness [%]	94.7	88.8	97.1
Redundancy	2.9	2.7	2.8
CC(1/2)		0.993	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	150mM D-glucose, 100mM HEPES/MOPS pH7.5, 40% Glycerol/PEG4000