4X3V
Crystal structure of human ribonucleotide reductase 1 bound to inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.880, 110.498, 216.721 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.361 - 3.700 |
| R-factor | 0.2381 |
| Rwork | 0.232 |
| R-free | 0.28920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hnc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.788 |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 4.040 |
| High resolution limit [Å] | 3.700 | 3.900 |
| Number of reflections | 19866 | |
| <I/σ(I)> | 32.6 | 4.5 |
| Completeness [%] | 95.6 | 97.9 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.9 | 298 | 0.1 M Tris-HCl pH 7.9, 0.2 M LiSO4, 19% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
