4X34
Crystal structure of the 53BP1 tandem tudor domain in complex with p53K381acK382me2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-08 |
| Detector | NOIR-1 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.891, 110.939, 96.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.458 - 1.801 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lgl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.156 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.640 |
| Number of reflections | 29571 | |
| <I/σ(I)> | 25.7 | 2.4 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 291 | 0.1 M sodium chloride and 4.0 M sodium formate |
