4WTV

Crystal structure of the phosphatidylinositol 4-kinase IIbeta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28272
Spacegroup name	P 41 21 2
Unit cell lengths	106.010, 106.010, 214.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.520 - 1.900
R-factor	0.2104
Rwork	0.209
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pla
RMSD bond length	0.010
RMSD bond angle	1.246
Data reduction software	XDS (January 10, 2014)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.520	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.100
Number of reflections	96773
<I/σ(I)>	17.42	1.8
Completeness [%]	100.0	99.94
Redundancy	17.6	17.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	100 mM MES/Imidazole pH = 6.5, 10% w/v PEG 4000, 20% v/v glycerol, 20 mM 1,6-hexanediol, 20 mM 1-butanol, 20 mM 1,2-propanediol, 20 mM 2-propanol, 20 mM 1,4-butanediol, 20 mM 1,3-propanediol