4WSN
Crystal structure of the COP9 signalosome, a P1 crystal form
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0004 |
Spacegroup name | P 1 |
Unit cell lengths | 150.638, 150.983, 336.720 |
Unit cell angles | 92.34, 92.62, 119.88 |
Refinement procedure
Resolution | 49.640 - 5.500 |
R-factor | 0.2548 |
Rwork | 0.254 |
R-free | 0.28210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d10 chains A-H |
RMSD bond length | 0.005 |
RMSD bond angle | 0.839 |
Data reduction software | XDS (January 10, 2014) |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.640 | 49.640 | 5.610 |
High resolution limit [Å] | 5.500 | 28.040 | 5.500 |
Rmerge | 0.072 | 0.025 | 1.329 |
Rpim | 0.065 | 0.023 | 1.205 |
Total number of observations | 153156 | 960 | 8216 |
Number of reflections | 79098 | ||
<I/σ(I)> | 5.4 | 20.2 | 0.6 |
Completeness [%] | 94.9 | 78.8 | 88.7 |
Redundancy | 1.9 | 1.9 | 1.9 |
CC(1/2) | 0.997 | 0.998 | 0.166 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | CRYSTALS GROWN BY VAPOR DIFFUSION BY MIXING 9.3 MG/ML PROTEIN IN 50 MM HEPES PH 7.4, 200 MM NACL, 2 MM EQUALLY WITH 12% PEG 6000, 100 MM TRISODIUM CITRATE PH 5.4, 0.1 M LI2SO4. |