4WRT
Crystal structure of Influenza B polymerase with bound vRNA promoter (form FluB2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 207.370, 207.370, 345.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.657 - 2.700 |
| R-factor | 0.1753 |
| Rwork | 0.173 |
| R-free | 0.21140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wsa |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.683 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.252 | 1.490 |
| Number of reflections | 120050 | |
| <I/σ(I)> | 10.3 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.1 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 281 | 1 M LiCl, 10% PEG 6000 and 0.1 M bicine pH 9.0 |
