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Promode Elastic




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    HEADER    TRANSFERASE/RNA                         25-OCT-14   4WRT              
    TITLE     CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND VRNA PROMOTER  
    TITLE    2 (FORM FLUB2)                                                         
    COMPND    MOL_ID: 1;                                                            
    COMPND   2 MOLECULE: INFLUENZA VIRUS POLYMERASE VRNA PROMOTER 3' END;           
    COMPND   3 CHAIN: R;                                                            
    COMPND   4 ENGINEERED: YES;                                                     
    COMPND   5 MOL_ID: 2;                                                           
    COMPND   6 MOLECULE: INFLUENZA VIRUS POLYMERASE VRNA PROMOTER 5' END;           
    COMPND   7 CHAIN: V;                                                            
    COMPND   8 ENGINEERED: YES;                                                     
    COMPND   9 MOL_ID: 3;                                                           
    COMPND  10 MOLECULE: PA;                                                        
    COMPND  11 CHAIN: A;                                                            
    COMPND  12 ENGINEERED: YES;                                                     
    COMPND  13 OTHER_DETAILS: N-TERMINAL EXTENSION CONTAINS HIS-TAG. C-TERMINAL     
    COMPND  14 EXTENSION CONTAINS LINKER AND RESIDUAL TEV CLEAVAGE SITE;            
    COMPND  15 MOL_ID: 4;                                                           
    COMPND  16 MOLECULE: RNA-DIRECTED RNA POLYMERASE CATALYTIC SUBUNIT;             
    COMPND  17 CHAIN: B;                                                            
    COMPND  18 EC: 2.7.7.48;                                                        
    COMPND  19 ENGINEERED: YES;                                                     
    COMPND  20 OTHER_DETAILS: N-TERMINAL EXTENSION CONTAINS LINKER. C-TERMINAL      
    COMPND  21 EXTENSION CONTAINS LINKER AND RESIDUAL TEV CLEAVAGE SITE;            
    COMPND  22 MOL_ID: 5;                                                           
    COMPND  23 MOLECULE: PB2;                                                       
    COMPND  24 CHAIN: C;                                                            
    COMPND  25 ENGINEERED: YES;                                                     
    COMPND  26 OTHER_DETAILS: N-TERMINAL EXTENSION CONTAINS LINKER. C-TERMINAL      
    COMPND  27 EXTENSION CONTAINS LINKER, STRP TAG AND RESIDUAL TEV CLEAVAGE SITE   
    SOURCE    MOL_ID: 1;                                                            
    SOURCE   2 SYNTHETIC: YES;                                                      
    SOURCE   3 ORGANISM_SCIENTIFIC: INFLUENZA B VIRUS;                              
    SOURCE   4 ORGANISM_TAXID: 11520;                                               
    SOURCE   5 MOL_ID: 2;                                                           
    SOURCE   6 SYNTHETIC: YES;                                                      
    SOURCE   7 ORGANISM_SCIENTIFIC: INFLUENZA B VIRUS;                              
    SOURCE   8 ORGANISM_TAXID: 11520;                                               
    SOURCE   9 MOL_ID: 3;                                                           
    SOURCE  10 ORGANISM_SCIENTIFIC: INFLUENZA B VIRUS;                              
    SOURCE  11 ORGANISM_TAXID: 289331;                                              
    SOURCE  12 STRAIN: B/MEMPHIS/13/03;                                             
    SOURCE  13 GENE: PA;                                                            
    SOURCE  14 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
    SOURCE  15 EXPRESSION_SYSTEM_COMMON: CABBAGE LOOPER;                            
    SOURCE  16 EXPRESSION_SYSTEM_TAXID: 7111;                                       
    SOURCE  17 EXPRESSION_SYSTEM_CELL_LINE: HIGHFIVE;                               
    SOURCE  18 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
    SOURCE  19 EXPRESSION_SYSTEM_PLASMID: PKL-PBAC;                                 
    SOURCE  20 MOL_ID: 4;                                                           
    SOURCE  21 ORGANISM_SCIENTIFIC: INFLUENZA B VIRUS;                              
    SOURCE  22 ORGANISM_TAXID: 289331;                                              
    SOURCE  23 STRAIN: B/MEMPHIS/13/03;                                             
    SOURCE  24 GENE: PB1;                                                           
    SOURCE  25 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
    SOURCE  26 EXPRESSION_SYSTEM_COMMON: CABBAGE LOOPER;                            
    SOURCE  27 EXPRESSION_SYSTEM_TAXID: 7111;                                       
    SOURCE  28 EXPRESSION_SYSTEM_CELL_LINE: HIGHFIVE;                               
    SOURCE  29 EXPRESSION_SYSTEM_PLASMID: PKL-PBAC;                                 
    SOURCE  30 MOL_ID: 5;                                                           
    SOURCE  31 ORGANISM_SCIENTIFIC: INFLUENZA B VIRUS;                              
    SOURCE  32 ORGANISM_TAXID: 289331;                                              
    SOURCE  33 STRAIN: B/MEMPHIS/13/03;                                             
    SOURCE  34 GENE: PB2;                                                           
    SOURCE  35 EXPRESSION_SYSTEM: TRICHOPLUSIA NI;                                  
    SOURCE  36 EXPRESSION_SYSTEM_COMMON: CABBAGE LOOPER;                            
    SOURCE  37 EXPRESSION_SYSTEM_TAXID: 7111;                                       
    SOURCE  38 EXPRESSION_SYSTEM_CELL_LINE: HIGHFIVE;                               
    SOURCE  39 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
    SOURCE  40 EXPRESSION_SYSTEM_PLASMID: PKL-PBAC                                  
    KEYWDS    TRANSFERASE-RNA COMPLEX                                               
    EXPDTA    X-RAY DIFFRACTION                                                     
    AUTHOR    S.REICH,D.GUILLIGAY,A.PFLUG,S.CUSACK                                  
    REVDAT   3   24-DEC-14 4WRT    1       JRNL                                     
    REVDAT   2   03-DEC-14 4WRT    1       SOURCE                                   
    REVDAT   1   19-NOV-14 4WRT    0                                                
    JRNL        AUTH   S.REICH,D.GUILLIGAY,A.PFLUG,H.MALET,I.BERGER,T.CREPIN,       
    JRNL        AUTH 2 D.HART,T.LUNARDI,M.NANAO,R.W.RUIGROK,S.CUSACK                
    JRNL        TITL   STRUCTURAL INSIGHT INTO CAP-SNATCHING AND RNA SYNTHESIS BY   
    JRNL        TITL 2 INFLUENZA POLYMERASE.                                        
    JRNL        REF    NATURE                        V. 516   361 2014              
    JRNL        REFN                   ESSN 1476-4687                               
    JRNL        PMID   25409151                                                     
    JRNL        DOI    10.1038/NATURE14009                                          
    REMARK   1                                                                      
    REMARK   1 REFERENCE 1                                                          
    REMARK   1  AUTH   A.PFLUG,D.GUILLIGAY,S.REICH,S.CUSACK                         
    REMARK   1  TITL   CRYSTAL STRUCTURE OF INFLUENZA A POLYMERASE BOUND TO THE     
    REMARK   1  TITL 2 VIRAL RNA PROMOTER                                           
    REMARK   1  REF    TO BE PUBLISHED                                              
    REMARK   1  REFN                                                                
    REMARK   2                                                                      
    REMARK   2 RESOLUTION.    2.70 ANGSTROMS.                                       
    REMARK   3                                                                      
    REMARK   3 REFINEMENT.                                                          
    REMARK   3   PROGRAM     : PHENIX (PHENIX.REFINE: DEV_1760)                     
    REMARK   3   AUTHORS     : PAUL ADAMS,PAVEL AFONINE,VINCENT CHEN,IAN            
    REMARK   3               : DAVIS,KRESHNA GOPAL,RALF GROSSE-KUNSTLEVE,           
    REMARK   3               : LI-WEI HUNG,ROBERT IMMORMINO,TOM IOERGER,            
    REMARK   3               : AIRLIE MCCOY,ERIK MCKEE,NIGEL MORIARTY,              
    REMARK   3               : REETAL PAI,RANDY READ,JANE RICHARDSON,               
    REMARK   3               : DAVID RICHARDSON,TOD ROMO,JIM SACCHETTINI,           
    REMARK   3               : NICHOLAS SAUTER,JACOB SMITH,LAURENT                  
    REMARK   3               : STORONI,TOM TERWILLIGER,PETER ZWART                  
    REMARK   3                                                                      
    REMARK   3    REFINEMENT TARGET : ML                                            
    REMARK   3                                                                      
    REMARK   3  DATA USED IN REFINEMENT.                                            
    REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.70                           
    REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 49.66                          
    REMARK   3   MIN(FOBS/SIGMA_FOBS)              : 1.360                          
    REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0                          
    REMARK   3   NUMBER OF REFLECTIONS             : 120037                         
    REMARK   3                                                                      
    REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
    REMARK   3   R VALUE     (WORKING + TEST SET) : 0.175                           
    REMARK   3   R VALUE            (WORKING SET) : 0.173                           
    REMARK   3   FREE R VALUE                     : 0.211                           
    REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 4.940                           
    REMARK   3   FREE R VALUE TEST SET COUNT      : 5930                            
    REMARK   3                                                                      
    REMARK   3  FIT TO DATA USED IN REFINEMENT (IN BINS).                           
    REMARK   3   BIN  RESOLUTION RANGE  COMPL.    NWORK NFREE   RWORK  RFREE        
    REMARK   3     1 49.6654 -  8.3766    1.00     4178   215  0.1844 0.1732        
    REMARK   3     2  8.3766 -  6.6538    1.00     3946   211  0.1565 0.1698        
    REMARK   3     3  6.6538 -  5.8142    1.00     3928   187  0.1581 0.1852        
    REMARK   3     4  5.8142 -  5.2833    1.00     3880   195  0.1495 0.1830        
    REMARK   3     5  5.2833 -  4.9049    1.00     3852   197  0.1387 0.1714        
    REMARK   3     6  4.9049 -  4.6160    1.00     3817   205  0.1239 0.1486        
    REMARK   3     7  4.6160 -  4.3849    1.00     3843   186  0.1270 0.1541        
    REMARK   3     8  4.3849 -  4.1942    1.00     3793   226  0.1299 0.1594        
    REMARK   3     9  4.1942 -  4.0328    1.00     3814   181  0.1319 0.1636        
    REMARK   3    10  4.0328 -  3.8937    1.00     3833   188  0.1376 0.1674        
    REMARK   3    11  3.8937 -  3.7720    1.00     3758   209  0.1534 0.1774        
    REMARK   3    12  3.7720 -  3.6642    1.00     3765   219  0.1590 0.2277        
    REMARK   3    13  3.6642 -  3.5677    1.00     3799   199  0.1706 0.2208        
    REMARK   3    14  3.5677 -  3.4807    1.00     3809   173  0.1760 0.2343        
    REMARK   3    15  3.4807 -  3.4016    1.00     3781   193  0.1877 0.2228        
    REMARK   3    16  3.4016 -  3.3292    1.00     3748   203  0.1941 0.2413        
    REMARK   3    17  3.3292 -  3.2627    1.00     3779   204  0.1967 0.2590        
    REMARK   3    18  3.2627 -  3.2011    1.00     3761   206  0.2007 0.2573        
    REMARK   3    19  3.2011 -  3.1439    1.00     3743   195  0.2079 0.2726        
    REMARK   3    20  3.1439 -  3.0907    1.00     3793   184  0.2074 0.2615        
    REMARK   3    21  3.0907 -  3.0408    1.00     3759   193  0.2132 0.2495        
    REMARK   3    22  3.0408 -  2.9940    1.00     3748   194  0.2161 0.2838        
    REMARK   3    23  2.9940 -  2.9500    1.00     3724   221  0.2256 0.2839        
    REMARK   3    24  2.9500 -  2.9085    1.00     3780   193  0.2297 0.2937        
    REMARK   3    25  2.9085 -  2.8692    1.00     3722   205  0.2346 0.2904        
    REMARK   3    26  2.8692 -  2.8319    1.00     3724   211  0.2416 0.3006        
    REMARK   3    27  2.8319 -  2.7965    1.00     3761   179  0.2356 0.2786        
    REMARK   3    28  2.7965 -  2.7628    1.00     3740   201  0.2460 0.2919        
    REMARK   3    29  2.7628 -  2.7307    1.00     3753   180  0.2679 0.3148        
    REMARK   3    30  2.7307 -  2.7000    1.00     3776   177  0.2893 0.3691        
    REMARK   3                                                                      
    REMARK   3  BULK SOLVENT MODELLING.                                             
    REMARK   3   METHOD USED        : FLAT BULK SOLVENT MODEL                       
    REMARK   3   SOLVENT RADIUS     : 1.11                                          
    REMARK   3   SHRINKAGE RADIUS   : 0.90                                          
    REMARK   3   K_SOL              : NULL                                          
    REMARK   3   B_SOL              : NULL                                          
    REMARK   3                                                                      
    REMARK   3  ERROR ESTIMATES.                                                    
    REMARK   3   COORDINATE ERROR (MAXIMUM-LIKELIHOOD BASED)     : 0.310            
    REMARK   3   PHASE ERROR (DEGREES, MAXIMUM-LIKELIHOOD BASED) : 20.880           
    REMARK   3                                                                      
    REMARK   3  B VALUES.                                                           
    REMARK   3   FROM WILSON PLOT           (A**2) : 40.60                          
    REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
    REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
    REMARK   3    B11 (A**2) : NULL                                                 
    REMARK   3    B22 (A**2) : NULL                                                 
    REMARK   3    B33 (A**2) : NULL                                                 
    REMARK   3    B12 (A**2) : NULL                                                 
    REMARK   3    B13 (A**2) : NULL                                                 
    REMARK   3    B23 (A**2) : NULL                                                 
    REMARK   3                                                                      
    REMARK   3  TWINNING INFORMATION.                                               
    REMARK   3   FRACTION: NULL                                                     
    REMARK   3   OPERATOR: NULL                                                     
    REMARK   3                                                                      
    REMARK   3  DEVIATIONS FROM IDEAL VALUES.                                       
    REMARK   3                 RMSD          COUNT                                  
    REMARK   3   BOND      :  0.003          14503                                  
    REMARK   3   ANGLE     :  0.683          19692                                  
    REMARK   3   CHIRALITY :  0.027           2186                                  
    REMARK   3   PLANARITY :  0.003           2422                                  
    REMARK   3   DIHEDRAL  : 13.728           5606                                  
    REMARK   3                                                                      
    REMARK   3  TLS DETAILS                                                         
    REMARK   3   NUMBER OF TLS GROUPS  : NULL                                       
    REMARK   3                                                                      
    REMARK   3  NCS DETAILS                                                         
    REMARK   3   NUMBER OF NCS GROUPS : NULL                                        
    REMARK   3                                                                      
    REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
    REMARK   4                                                                      
    REMARK   4 4WRT COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
    REMARK 100                                                                      
    REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 27-OCT-14.                  
    REMARK 100 THE DEPOSITION ID IS D_1000204393.                                   
    REMARK 200                                                                      
    REMARK 200 EXPERIMENTAL DETAILS                                                 
    REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
    REMARK 200  DATE OF DATA COLLECTION        : 14-JUL-14                          
    REMARK 200  TEMPERATURE           (KELVIN) : 100                                
    REMARK 200  PH                             : 9.0                                
    REMARK 200  NUMBER OF CRYSTALS USED        : NULL                               
    REMARK 200                                                                      
    REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
    REMARK 200  RADIATION SOURCE               : ESRF                               
    REMARK 200  BEAMLINE                       : ID23-1                             
    REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
    REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
    REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9792                             
    REMARK 200  MONOCHROMATOR                  : NULL                               
    REMARK 200  OPTICS                         : NULL                               
    REMARK 200                                                                      
    REMARK 200  DETECTOR TYPE                  : PIXEL                              
    REMARK 200  DETECTOR MANUFACTURER          : DECTRIS PILATUS 6M-F               
    REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
    REMARK 200  DATA SCALING SOFTWARE          : XSCALE                             
    REMARK 200                                                                      
    REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 120050                             
    REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.700                              
    REMARK 200  RESOLUTION RANGE LOW       (A) : 50.000                             
    REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
    REMARK 200                                                                      
    REMARK 200 OVERALL.                                                             
    REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.9                               
    REMARK 200  DATA REDUNDANCY                : 10.10                              
    REMARK 200  R MERGE                    (I) : 0.25200                            
    REMARK 200  R SYM                      (I) : NULL                               
    REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 10.3000                            
    REMARK 200                                                                      
    REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
    REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.70                     
    REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.80                     
    REMARK 200  COMPLETENESS FOR SHELL     (%) : 100.0                              
    REMARK 200  DATA REDUNDANCY IN SHELL       : 10.30                              
    REMARK 200  R MERGE FOR SHELL          (I) : 1.49000                            
    REMARK 200  R SYM FOR SHELL            (I) : NULL                               
    REMARK 200  <I/SIGMA(I)> FOR SHELL         : 1.900                              
    REMARK 200                                                                      
    REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
    REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
    REMARK 200 SOFTWARE USED: PHASER                                                
    REMARK 200 STARTING MODEL: 4WSA                                                 
    REMARK 200                                                                      
    REMARK 200 REMARK: PLATE                                                        
    REMARK 280                                                                      
    REMARK 280 CRYSTAL                                                              
    REMARK 280 SOLVENT CONTENT, VS   (%): 68.70                                     
    REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.93                     
    REMARK 280                                                                      
    REMARK 280 CRYSTALLIZATION CONDITIONS: 1 M LICL, 10% PEG 6000 AND 0.1 M         
    REMARK 280  BICINE PH 9.0                                                       
    REMARK 290                                                                      
    REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
    REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 63 2 2                         
    REMARK 290                                                                      
    REMARK 290      SYMOP   SYMMETRY                                                
    REMARK 290     NNNMMM   OPERATOR                                                
    REMARK 290       1555   X,Y,Z                                                   
    REMARK 290       2555   -Y,X-Y,Z                                                
    REMARK 290       3555   -X+Y,-X,Z                                               
    REMARK 290       4555   -X,-Y,Z+1/2                                             
    REMARK 290       5555   Y,-X+Y,Z+1/2                                            
    REMARK 290       6555   X-Y,X,Z+1/2                                             
    REMARK 290       7555   Y,X,-Z                                                  
    REMARK 290       8555   X-Y,-Y,-Z                                               
    REMARK 290       9555   -X,-X+Y,-Z                                              
    REMARK 290      10555   -Y,-X,-Z+1/2                                            
    REMARK 290      11555   -X+Y,Y,-Z+1/2                                           
    REMARK 290      12555   X,X-Y,-Z+1/2                                            
    REMARK 290                                                                      
    REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
    REMARK 290           MMM -> TRANSLATION VECTOR                                  
    REMARK 290                                                                      
    REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
    REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
    REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
    REMARK 290 RELATED MOLECULES.                                                   
    REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
    REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
    REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
    REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
    REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
    REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000        0.00000            
    REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
    REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000      172.84500            
    REMARK 290   SMTRY1   5  0.500000  0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2   5 -0.866025  0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000      172.84500            
    REMARK 290   SMTRY1   6  0.500000 -0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2   6  0.866025  0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000      172.84500            
    REMARK 290   SMTRY1   7 -0.500000  0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2   7  0.866025  0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000        0.00000            
    REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000        0.00000            
    REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000        0.00000            
    REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
    REMARK 290   SMTRY1   9 -0.500000 -0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2   9 -0.866025  0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3   9  0.000000  0.000000 -1.000000        0.00000            
    REMARK 290   SMTRY1  10  0.500000 -0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2  10 -0.866025 -0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3  10  0.000000  0.000000 -1.000000      172.84500            
    REMARK 290   SMTRY1  11 -1.000000  0.000000  0.000000        0.00000            
    REMARK 290   SMTRY2  11  0.000000  1.000000  0.000000        0.00000            
    REMARK 290   SMTRY3  11  0.000000  0.000000 -1.000000      172.84500            
    REMARK 290   SMTRY1  12  0.500000  0.866025  0.000000        0.00000            
    REMARK 290   SMTRY2  12  0.866025 -0.500000  0.000000        0.00000            
    REMARK 290   SMTRY3  12  0.000000  0.000000 -1.000000      172.84500            
    REMARK 290                                                                      
    REMARK 290 REMARK: NULL                                                         
    REMARK 300                                                                      
    REMARK 300 BIOMOLECULE: 1                                                       
    REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
    REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
    REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
    REMARK 300 BURIED SURFACE AREA.                                                 
    REMARK 350                                                                      
    REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
    REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
    REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
    REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
    REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
    REMARK 350                                                                      
    REMARK 350 BIOMOLECULE: 1                                                       
    REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC                        
    REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: PENTAMERIC                 
    REMARK 350 SOFTWARE USED: PISA                                                  
    REMARK 350 TOTAL BURIED SURFACE AREA: 39100 ANGSTROM**2                         
    REMARK 350 SURFACE AREA OF THE COMPLEX: 67500 ANGSTROM**2                       
    REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -250.0 KCAL/MOL                       
    REMARK 350 APPLY THE FOLLOWING TO CHAINS: R, V, A, B, C                         
    REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
    REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
    REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
    REMARK 375                                                                      
    REMARK 375 SPECIAL POSITION                                                     
    REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
    REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
    REMARK 375 POSITIONS.                                                           
    REMARK 375                                                                      
    REMARK 375 ATOM RES CSSEQI                                                      
    REMARK 375      HOH C 801  LIES ON A SPECIAL POSITION.                          
    REMARK 465                                                                      
    REMARK 465 MISSING RESIDUES                                                     
    REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
    REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
    REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
    REMARK 465                                                                      
    REMARK 465   M RES C SSSEQI                                                     
    REMARK 465       U R     1                                                      
    REMARK 465       A R     2                                                      
    REMARK 465       U R     3                                                      
    REMARK 465     GLY A   -13                                                      
    REMARK 465     SER A   -12                                                      
    REMARK 465     HIS A   -11                                                      
    REMARK 465     HIS A   -10                                                      
    REMARK 465     HIS A    -9                                                      
    REMARK 465     HIS A    -8                                                      
    REMARK 465     HIS A    -7                                                      
    REMARK 465     HIS A    -6                                                      
    REMARK 465     HIS A    -5                                                      
    REMARK 465     HIS A    -4                                                      
    REMARK 465     GLY A    -3                                                      
    REMARK 465     SER A    -2                                                      
    REMARK 465     GLY A    -1                                                      
    REMARK 465     LEU A    64                                                      
    REMARK 465     GLU A    65                                                      
    REMARK 465     GLY A    66                                                      
    REMARK 465     GLN A    67                                                      
    REMARK 465     GLY A    68                                                      
    REMARK 465     LYS A    69                                                      
    REMARK 465     GLU A    70                                                      
    REMARK 465     GLN A    71                                                      
    REMARK 465     LEU A   717                                                      
    REMARK 465     GLU A   718                                                      
    REMARK 465     SER A   719                                                      
    REMARK 465     VAL A   720                                                      
    REMARK 465     ASP A   721                                                      
    REMARK 465     GLU A   722                                                      
    REMARK 465     ILE A   723                                                      
    REMARK 465     MET A   724                                                      
    REMARK 465     ASP A   725                                                      
    REMARK 465     GLU A   726                                                      
    REMARK 465     GLY A   727                                                      
    REMARK 465     SER A   728                                                      
    REMARK 465     GLY A   729                                                      
    REMARK 465     SER A   730                                                      
    REMARK 465     GLY A   731                                                      
    REMARK 465     GLU A   732                                                      
    REMARK 465     ASN A   733                                                      
    REMARK 465     LEU A   734                                                      
    REMARK 465     TYR A   735                                                      
    REMARK 465     PHE A   736                                                      
    REMARK 465     GLN A   737                                                      
    REMARK 465     GLY B    -8                                                      
    REMARK 465     SER B    -7                                                      
    REMARK 465     GLY B    -6                                                      
    REMARK 465     SER B    -5                                                      
    REMARK 465     GLY B    -4                                                      
    REMARK 465     SER B    -3                                                      
    REMARK 465     GLY B    -2                                                      
    REMARK 465     SER B    -1                                                      
    REMARK 465     GLY B     0                                                      
    REMARK 465     PRO B   646                                                      
    REMARK 465     ALA B   647                                                      
    REMARK 465     HIS B   648                                                      
    REMARK 465     GLY B   649                                                      
    REMARK 465     PRO B   650                                                      
    REMARK 465     VAL B   651                                                      
    REMARK 465     LYS B   652                                                      
    REMARK 465     GLY B   750                                                      
    REMARK 465     TYR B   751                                                      
    REMARK 465     ILE B   752                                                      
    REMARK 465     GLY B   753                                                      
    REMARK 465     SER B   754                                                      
    REMARK 465     GLY B   755                                                      
    REMARK 465     SER B   756                                                      
    REMARK 465     GLY B   757                                                      
    REMARK 465     GLU B   758                                                      
    REMARK 465     ASN B   759                                                      
    REMARK 465     LEU B   760                                                      
    REMARK 465     TYR B   761                                                      
    REMARK 465     PHE B   762                                                      
    REMARK 465     GLN B   763                                                      
    REMARK 465     GLY C    -8                                                      
    REMARK 465     SER C    -7                                                      
    REMARK 465     GLY C    -6                                                      
    REMARK 465     SER C    -5                                                      
    REMARK 465     GLY C    -4                                                      
    REMARK 465     SER C    -3                                                      
    REMARK 465     GLY C    -2                                                      
    REMARK 465     SER C    -1                                                      
    REMARK 465     GLY C   250                                                      
    REMARK 465     ASN C   251                                                      
    REMARK 465     LYS C   252                                                      
    REMARK 465     LEU C   253                                                      
    REMARK 465     THR C   254                                                      
    REMARK 465     GLU C   255                                                      
    REMARK 465     SER C   256                                                      
    REMARK 465     ARG C   257                                                      
    REMARK 465     SER C   258                                                      
    REMARK 465     GLN C   259                                                      
    REMARK 465     SER C   260                                                      
    REMARK 465     MET C   261                                                      
    REMARK 465     ILE C   262                                                      
    REMARK 465     VAL C   263                                                      
    REMARK 465     ALA C   264                                                      
    REMARK 465     CYS C   265                                                      
    REMARK 465     ARG C   266                                                      
    REMARK 465     LYS C   267                                                      
    REMARK 465     ILE C   268                                                      
    REMARK 465     ILE C   269                                                      
    REMARK 465     ARG C   270                                                      
    REMARK 465     ARG C   271                                                      
    REMARK 465     SER C   272                                                      
    REMARK 465     ILE C   273                                                      
    REMARK 465     VAL C   274                                                      
    REMARK 465     ALA C   275                                                      
    REMARK 465     SER C   276                                                      
    REMARK 465     ASN C   277                                                      
    REMARK 465     PRO C   278                                                      
    REMARK 465     LEU C   279                                                      
    REMARK 465     GLU C   280                                                      
    REMARK 465     LEU C   281                                                      
    REMARK 465     ALA C   282                                                      
    REMARK 465     VAL C   283                                                      
    REMARK 465     GLU C   284                                                      
    REMARK 465     ILE C   285                                                      
    REMARK 465     ALA C   286                                                      
    REMARK 465     ASN C   287                                                      
    REMARK 465     LYS C   288                                                      
    REMARK 465     THR C   289                                                      
    REMARK 465     VAL C   290                                                      
    REMARK 465     ILE C   291                                                      
    REMARK 465     ASP C   292                                                      
    REMARK 465     THR C   293                                                      
    REMARK 465     GLU C   294                                                      
    REMARK 465     PRO C   295                                                      
    REMARK 465     LEU C   296                                                      
    REMARK 465     LYS C   297                                                      
    REMARK 465     SER C   298                                                      
    REMARK 465     CYS C   299                                                      
    REMARK 465     LEU C   300                                                      
    REMARK 465     ALA C   301                                                      
    REMARK 465     ALA C   302                                                      
    REMARK 465     ILE C   303                                                      
    REMARK 465     ASP C   304                                                      
    REMARK 465     GLY C   305                                                      
    REMARK 465     GLY C   306                                                      
    REMARK 465     ASP C   307                                                      
    REMARK 465     VAL C   308                                                      
    REMARK 465     ALA C   309                                                      
    REMARK 465     CYS C   310                                                      
    REMARK 465     ASP C   311                                                      
    REMARK 465     ILE C   312                                                      
    REMARK 465     ILE C   313                                                      
    REMARK 465     ARG C   314                                                      
    REMARK 465     ALA C   315                                                      
    REMARK 465     ALA C   316                                                      
    REMARK 465     LEU C   317                                                      
    REMARK 465     GLY C   318                                                      
    REMARK 465     LEU C   319                                                      
    REMARK 465     LYS C   320                                                      
    REMARK 465     ILE C   321                                                      
    REMARK 465     ARG C   322                                                      
    REMARK 465     GLN C   323                                                      
    REMARK 465     ARG C   324                                                      
    REMARK 465     GLN C   325                                                      
    REMARK 465     ARG C   326                                                      
    REMARK 465     PHE C   327                                                      
    REMARK 465     GLY C   328                                                      
    REMARK 465     ARG C   329                                                      
    REMARK 465     LEU C   330                                                      
    REMARK 465     GLU C   331                                                      
    REMARK 465     LEU C   332                                                      
    REMARK 465     LYS C   333                                                      
    REMARK 465     ARG C   334                                                      
    REMARK 465     ILE C   335                                                      
    REMARK 465     SER C   336                                                      
    REMARK 465     GLY C   337                                                      
    REMARK 465     ARG C   338                                                      
    REMARK 465     GLY C   339                                                      
    REMARK 465     PHE C   340                                                      
    REMARK 465     LYS C   341                                                      
    REMARK 465     ASN C   342                                                      
    REMARK 465     ASP C   343                                                      
    REMARK 465     GLU C   344                                                      
    REMARK 465     GLU C   345                                                      
    REMARK 465     ILE C   346                                                      
    REMARK 465     LEU C   347                                                      
    REMARK 465     ILE C   348                                                      
    REMARK 465     GLY C   349                                                      
    REMARK 465     ASN C   350                                                      
    REMARK 465     GLY C   351                                                      
    REMARK 465     THR C   352                                                      
    REMARK 465     ILE C   353                                                      
    REMARK 465     GLN C   354                                                      
    REMARK 465     LYS C   355                                                      
    REMARK 465     ILE C   356                                                      
    REMARK 465     GLY C   357                                                      
    REMARK 465     ILE C   358                                                      
    REMARK 465     TRP C   359                                                      
    REMARK 465     ASP C   360                                                      
    REMARK 465     GLY C   361                                                      
    REMARK 465     GLU C   362                                                      
    REMARK 465     GLU C   363                                                      
    REMARK 465     GLU C   364                                                      
    REMARK 465     PHE C   365                                                      
    REMARK 465     HIS C   366                                                      
    REMARK 465     VAL C   367                                                      
    REMARK 465     ARG C   368                                                      
    REMARK 465     CYS C   369                                                      
    REMARK 465     GLY C   370                                                      
    REMARK 465     GLU C   371                                                      
    REMARK 465     CYS C   372                                                      
    REMARK 465     ARG C   373                                                      
    REMARK 465     GLY C   374                                                      
    REMARK 465     ILE C   375                                                      
    REMARK 465     LEU C   376                                                      
    REMARK 465     LYS C   377                                                      
    REMARK 465     LYS C   378                                                      
    REMARK 465     SER C   379                                                      
    REMARK 465     LYS C   380                                                      
    REMARK 465     MET C   381                                                      
    REMARK 465     LYS C   382                                                      
    REMARK 465     LEU C   383                                                      
    REMARK 465     GLU C   384                                                      
    REMARK 465     LYS C   385                                                      
    REMARK 465     LEU C   386                                                      
    REMARK 465     LEU C   387                                                      
    REMARK 465     ILE C   388                                                      
    REMARK 465     ASN C   389                                                      
    REMARK 465     SER C   390                                                      
    REMARK 465     ALA C   391                                                      
    REMARK 465     LYS C   392                                                      
    REMARK 465     LYS C   393                                                      
    REMARK 465     GLU C   394                                                      
    REMARK 465     ASP C   395                                                      
    REMARK 465     MET C   396                                                      
    REMARK 465     ARG C   397                                                      
    REMARK 465     ASP C   398                                                      
    REMARK 465     LEU C   399                                                      
    REMARK 465     ILE C   400                                                      
    REMARK 465     ILE C   401                                                      
    REMARK 465     LEU C   402                                                      
    REMARK 465     CYS C   403                                                      
    REMARK 465     MET C   404                                                      
    REMARK 465     VAL C   405                                                      
    REMARK 465     PHE C   406                                                      
    REMARK 465     SER C   407                                                      
    REMARK 465     GLN C   408                                                      
    REMARK 465     ASP C   409                                                      
    REMARK 465     THR C   410                                                      
    REMARK 465     ARG C   411                                                      
    REMARK 465     MET C   412                                                      
    REMARK 465     PHE C   413                                                      
    REMARK 465     GLN C   414                                                      
    REMARK 465     GLY C   415                                                      
    REMARK 465     VAL C   416                                                      
    REMARK 465     ARG C   417                                                      
    REMARK 465     GLY C   418                                                      
    REMARK 465     GLU C   419                                                      
    REMARK 465     ILE C   420                                                      
    REMARK 465     ASN C   421                                                      
    REMARK 465     PHE C   422                                                      
    REMARK 465     LEU C   423                                                      
    REMARK 465     ASN C   424                                                      
    REMARK 465     ARG C   425                                                      
    REMARK 465     ALA C   426                                                      
    REMARK 465     GLY C   427                                                      
    REMARK 465     GLN C   428                                                      
    REMARK 465     LEU C   429                                                      
    REMARK 465     LEU C   430                                                      
    REMARK 465     SER C   431                                                      
    REMARK 465     PRO C   432                                                      
    REMARK 465     MET C   433                                                      
    REMARK 465     TYR C   434                                                      
    REMARK 465     GLN C   435                                                      
    REMARK 465     LEU C   436                                                      
    REMARK 465     GLN C   437                                                      
    REMARK 465     ARG C   438                                                      
    REMARK 465     TYR C   439                                                      
    REMARK 465     PHE C   440                                                      
    REMARK 465     LEU C   441                                                      
    REMARK 465     ASN C   442                                                      
    REMARK 465     ARG C   443                                                      
    REMARK 465     SER C   444                                                      
    REMARK 465     ASN C   445                                                      
    REMARK 465     ASP C   446                                                      
    REMARK 465     LEU C   447                                                      
    REMARK 465     PHE C   448                                                      
    REMARK 465     ASP C   449                                                      
    REMARK 465     GLN C   450                                                      
    REMARK 465     TRP C   451                                                      
    REMARK 465     GLY C   452                                                      
    REMARK 465     TYR C   453                                                      
    REMARK 465     GLU C   454                                                      
    REMARK 465     GLU C   455                                                      
    REMARK 465     SER C   456                                                      
    REMARK 465     PRO C   457                                                      
    REMARK 465     LYS C   458                                                      
    REMARK 465     ALA C   459                                                      
    REMARK 465     SER C   460                                                      
    REMARK 465     GLU C   461                                                      
    REMARK 465     LEU C   462                                                      
    REMARK 465     HIS C   463                                                      
    REMARK 465     GLY C   464                                                      
    REMARK 465     ILE C   465                                                      
    REMARK 465     ASN C   466                                                      
    REMARK 465     GLU C   467                                                      
    REMARK 465     SER C   468                                                      
    REMARK 465     MET C   469                                                      
    REMARK 465     ASN C   470                                                      
    REMARK 465     ALA C   471                                                      
    REMARK 465     SER C   472                                                      
    REMARK 465     ASP C   473                                                      
    REMARK 465     TYR C   474                                                      
    REMARK 465     THR C   475                                                      
    REMARK 465     LEU C   476                                                      
    REMARK 465     LYS C   477                                                      
    REMARK 465     GLY C   478                                                      
    REMARK 465     VAL C   479                                                      
    REMARK 465     VAL C   480                                                      
    REMARK 465     VAL C   481                                                      
    REMARK 465     THR C   482                                                      
    REMARK 465     ARG C   483                                                      
    REMARK 465     ASN C   484                                                      
    REMARK 465     VAL C   485                                                      
    REMARK 465     ILE C   486                                                      
    REMARK 465     ASP C   487                                                      
    REMARK 465     ASP C   488                                                      
    REMARK 465     PHE C   489                                                      
    REMARK 465     SER C   490                                                      
    REMARK 465     SER C   491                                                      
    REMARK 465     THR C   492                                                      
    REMARK 465     GLU C   493                                                      
    REMARK 465     THR C   494                                                      
    REMARK 465     GLU C   495                                                      
    REMARK 465     LYS C   496                                                      
    REMARK 465     VAL C   497                                                      
    REMARK 465     SER C   498                                                      
    REMARK 465     ILE C   499                                                      
    REMARK 465     THR C   500                                                      
    REMARK 465     LYS C   501                                                      
    REMARK 465     ASN C   502                                                      
    REMARK 465     LEU C   503                                                      
    REMARK 465     SER C   504                                                      
    REMARK 465     LEU C   505                                                      
    REMARK 465     ILE C   506                                                      
    REMARK 465     LYS C   507                                                      
    REMARK 465     ARG C   508                                                      
    REMARK 465     THR C   509                                                      
    REMARK 465     GLY C   510                                                      
    REMARK 465     GLU C   511                                                      
    REMARK 465     VAL C   512                                                      
    REMARK 465     ILE C   513                                                      
    REMARK 465     MET C   514                                                      
    REMARK 465     GLY C   515                                                      
    REMARK 465     ALA C   516                                                      
    REMARK 465     ASN C   517                                                      
    REMARK 465     ASP C   518                                                      
    REMARK 465     VAL C   519                                                      
    REMARK 465     SER C   520                                                      
    REMARK 465     GLU C   521                                                      
    REMARK 465     LEU C   522                                                      
    REMARK 465     GLU C   523                                                      
    REMARK 465     SER C   524                                                      
    REMARK 465     GLN C   525                                                      
    REMARK 465     ALA C   526                                                      
    REMARK 465     GLN C   527                                                      
    REMARK 465     LEU C   528                                                      
    REMARK 465     MET C   529                                                      
    REMARK 465     ILE C   530                                                      
    REMARK 465     THR C   531                                                      
    REMARK 465     TYR C   532                                                      
    REMARK 465     ASP C   533                                                      
    REMARK 465     THR C   534                                                      
    REMARK 465     PRO C   535                                                      
    REMARK 465     LYS C   536                                                      
    REMARK 465     MET C   537                                                      
    REMARK 465     TRP C   538                                                      
    REMARK 465     GLU C   539                                                      
    REMARK 465     MET C   540                                                      
    REMARK 465     GLY C   541                                                      
    REMARK 465     THR C   542                                                      
    REMARK 465     THR C   543                                                      
    REMARK 465     LYS C   544                                                      
    REMARK 465     GLU C   545                                                      
    REMARK 465     LEU C   546                                                      
    REMARK 465     VAL C   547                                                      
    REMARK 465     GLN C   548                                                      
    REMARK 465     ASN C   549                                                      
    REMARK 465     THR C   550                                                      
    REMARK 465     TYR C   551                                                      
    REMARK 465     GLN C   552                                                      
    REMARK 465     TRP C   553                                                      
    REMARK 465     VAL C   554                                                      
    REMARK 465     LEU C   555                                                      
    REMARK 465     LYS C   556                                                      
    REMARK 465     ASN C   557                                                      
    REMARK 465     LEU C   558                                                      
    REMARK 465     VAL C   559                                                      
    REMARK 465     THR C   560                                                      
    REMARK 465     LEU C   561                                                      
    REMARK 465     LYS C   562                                                      
    REMARK 465     ALA C   563                                                      
    REMARK 465     GLN C   564                                                      
    REMARK 465     PHE C   565                                                      
    REMARK 465     LEU C   566                                                      
    REMARK 465     LEU C   567                                                      
    REMARK 465     GLY C   568                                                      
    REMARK 465     LYS C   569                                                      
    REMARK 465     GLU C   570                                                      
    REMARK 465     ASP C   571                                                      
    REMARK 465     MET C   572                                                      
    REMARK 465     PHE C   573                                                      
    REMARK 465     GLN C   574                                                      
    REMARK 465     TRP C   575                                                      
    REMARK 465     ASP C   576                                                      
    REMARK 465     ALA C   577                                                      
    REMARK 465     PHE C   578                                                      
    REMARK 465     GLU C   579                                                      
    REMARK 465     ALA C   580                                                      
    REMARK 465     PHE C   581                                                      
    REMARK 465     GLU C   582                                                      
    REMARK 465     SER C   583                                                      
    REMARK 465     ILE C   584                                                      
    REMARK 465     ILE C   585                                                      
    REMARK 465     PRO C   586                                                      
    REMARK 465     GLN C   587                                                      
    REMARK 465     LYS C   588                                                      
    REMARK 465     MET C   589                                                      
    REMARK 465     ALA C   590                                                      
    REMARK 465     GLY C   591                                                      
    REMARK 465     GLN C   592                                                      
    REMARK 465     TYR C   593                                                      
    REMARK 465     SER C   594                                                      
    REMARK 465     GLY C   595                                                      
    REMARK 465     PHE C   596                                                      
    REMARK 465     ALA C   597                                                      
    REMARK 465     ARG C   598                                                      
    REMARK 465     ALA C   599                                                      
    REMARK 465     VAL C   600                                                      
    REMARK 465     LEU C   601                                                      
    REMARK 465     LYS C   602                                                      
    REMARK 465     GLN C   603                                                      
    REMARK 465     MET C   604                                                      
    REMARK 465     ARG C   605                                                      
    REMARK 465     ASP C   606                                                      
    REMARK 465     GLN C   607                                                      
    REMARK 465     GLU C   608                                                      
    REMARK 465     VAL C   609                                                      
    REMARK 465     MET C   610                                                      
    REMARK 465     LYS C   611                                                      
    REMARK 465     THR C   612                                                      
    REMARK 465     ASP C   613                                                      
    REMARK 465     GLN C   614                                                      
    REMARK 465     PHE C   615                                                      
    REMARK 465     ILE C   616                                                      
    REMARK 465     LYS C   617                                                      
    REMARK 465     LEU C   618                                                      
    REMARK 465     LEU C   619                                                      
    REMARK 465     PRO C   620                                                      
    REMARK 465     PHE C   621                                                      
    REMARK 465     CYS C   622                                                      
    REMARK 465     PHE C   623                                                      
    REMARK 465     SER C   624                                                      
    REMARK 465     PRO C   625                                                      
    REMARK 465     PRO C   626                                                      
    REMARK 465     LYS C   627                                                      
    REMARK 465     LEU C   628                                                      
    REMARK 465     ARG C   629                                                      
    REMARK 465     SER C   630                                                      
    REMARK 465     ASN C   631                                                      
    REMARK 465     GLY C   632                                                      
    REMARK 465     GLU C   633                                                      
    REMARK 465     PRO C   634                                                      
    REMARK 465     TYR C   635                                                      
    REMARK 465     GLN C   636                                                      
    REMARK 465     PHE C   637                                                      
    REMARK 465     LEU C   638                                                      
    REMARK 465     LYS C   639                                                      
    REMARK 465     LEU C   640                                                      
    REMARK 465     VAL C   641                                                      
    REMARK 465     LEU C   642                                                      
    REMARK 465     LYS C   643                                                      
    REMARK 465     GLY C   644                                                      
    REMARK 465     GLY C   645                                                      
    REMARK 465     GLY C   646                                                      
    REMARK 465     GLU C   647                                                      
    REMARK 465     ASN C   648                                                      
    REMARK 465     PHE C   649                                                      
    REMARK 465     ILE C   650                                                      
    REMARK 465     GLU C   651                                                      
    REMARK 465     VAL C   652                                                      
    REMARK 465     ARG C   653                                                      
    REMARK 465     LYS C   654                                                      
    REMARK 465     GLY C   655                                                      
    REMARK 465     SER C   656                                                      
    REMARK 465     PRO C   657                                                      
    REMARK 465     LEU C   658                                                      
    REMARK 465     PHE C   659                                                      
    REMARK 465     SER C   660                                                      
    REMARK 465     TYR C   661                                                      
    REMARK 465     ASN C   662                                                      
    REMARK 465     PRO C   663                                                      
    REMARK 465     GLN C   664                                                      
    REMARK 465     THR C   665                                                      
    REMARK 465     GLU C   666                                                      
    REMARK 465     VAL C   667                                                      
    REMARK 465     LEU C   668                                                      
    REMARK 465     THR C   669                                                      
    REMARK 465     ILE C   670                                                      
    REMARK 465     CYS C   671                                                      
    REMARK 465     GLY C   672                                                      
    REMARK 465     ARG C   673                                                      
    REMARK 465     MET C   674                                                      
    REMARK 465     MET C   675                                                      
    REMARK 465     SER C   676                                                      
    REMARK 465     LEU C   677                                                      
    REMARK 465     LYS C   678                                                      
    REMARK 465     GLY C   679                                                      
    REMARK 465     LYS C   680                                                      
    REMARK 465     ILE C   681                                                      
    REMARK 465     GLU C   682                                                      
    REMARK 465     ASP C   683                                                      
    REMARK 465     GLU C   684                                                      
    REMARK 465     GLU C   685                                                      
    REMARK 465     ARG C   686                                                      
    REMARK 465     ASN C   687                                                      
    REMARK 465     ARG C   688                                                      
    REMARK 465     SER C   689                                                      
    REMARK 465     MET C   690                                                      
    REMARK 465     GLY C   691                                                      
    REMARK 465     ASN C   692                                                      
    REMARK 465     ALA C   693                                                      
    REMARK 465     VAL C   694                                                      
    REMARK 465     LEU C   695                                                      
    REMARK 465     ALA C   696                                                      
    REMARK 465     GLY C   697                                                      
    REMARK 465     PHE C   698                                                      
    REMARK 465     LEU C   699                                                      
    REMARK 465     VAL C   700                                                      
    REMARK 465     SER C   701                                                      
    REMARK 465     GLY C   702                                                      
    REMARK 465     LYS C   703                                                      
    REMARK 465     TYR C   704                                                      
    REMARK 465     ASP C   705                                                      
    REMARK 465     PRO C   706                                                      
    REMARK 465     ASP C   707                                                      
    REMARK 465     LEU C   708                                                      
    REMARK 465     GLY C   709                                                      
    REMARK 465     ASP C   710                                                      
    REMARK 465     PHE C   711                                                      
    REMARK 465     LYS C   712                                                      
    REMARK 465     THR C   713                                                      
    REMARK 465     ILE C   714                                                      
    REMARK 465     GLU C   715                                                      
    REMARK 465     GLU C   716                                                      
    REMARK 465     LEU C   717                                                      
    REMARK 465     GLU C   718                                                      
    REMARK 465     LYS C   719                                                      
    REMARK 465     LEU C   720                                                      
    REMARK 465     LYS C   721                                                      
    REMARK 465     PRO C   722                                                      
    REMARK 465     GLY C   723                                                      
    REMARK 465     GLU C   724                                                      
    REMARK 465     LYS C   725                                                      
    REMARK 465     ALA C   726                                                      
    REMARK 465     ASN C   727                                                      
    REMARK 465     ILE C   728                                                      
    REMARK 465     LEU C   729                                                      
    REMARK 465     LEU C   730                                                      
    REMARK 465     TYR C   731                                                      
    REMARK 465     GLN C   732                                                      
    REMARK 465     GLY C   733                                                      
    REMARK 465     LYS C   734                                                      
    REMARK 465     PRO C   735                                                      
    REMARK 465     VAL C   736                                                      
    REMARK 465     LYS C   737                                                      
    REMARK 465     VAL C   738                                                      
    REMARK 465     VAL C   739                                                      
    REMARK 465     LYS C   740                                                      
    REMARK 465     ARG C   741                                                      
    REMARK 465     LYS C   742                                                      
    REMARK 465     ARG C   743                                                      
    REMARK 465     TYR C   744                                                      
    REMARK 465     SER C   745                                                      
    REMARK 465     ALA C   746                                                      
    REMARK 465     LEU C   747                                                      
    REMARK 465     SER C   748                                                      
    REMARK 465     ASN C   749                                                      
    REMARK 465     ASP C   750                                                      
    REMARK 465     ILE C   751                                                      
    REMARK 465     SER C   752                                                      
    REMARK 465     GLN C   753                                                      
    REMARK 465     GLY C   754                                                      
    REMARK 465     ILE C   755                                                      
    REMARK 465     LYS C   756                                                      
    REMARK 465     ARG C   757                                                      
    REMARK 465     GLN C   758                                                      
    REMARK 465     ARG C   759                                                      
    REMARK 465     MET C   760                                                      
    REMARK 465     THR C   761                                                      
    REMARK 465     VAL C   762                                                      
    REMARK 465     GLU C   763                                                      
    REMARK 465     SER C   764                                                      
    REMARK 465     MET C   765                                                      
    REMARK 465     GLY C   766                                                      
    REMARK 465     TRP C   767                                                      
    REMARK 465     ALA C   768                                                      
    REMARK 465     LEU C   769                                                      
    REMARK 465     SER C   770                                                      
    REMARK 465     GLY C   771                                                      
    REMARK 465     TRP C   772                                                      
    REMARK 465     SER C   773                                                      
    REMARK 465     HIS C   774                                                      
    REMARK 465     PRO C   775                                                      
    REMARK 465     GLN C   776                                                      
    REMARK 465     PHE C   777                                                      
    REMARK 465     GLU C   778                                                      
    REMARK 465     LYS C   779                                                      
    REMARK 465     GLY C   780                                                      
    REMARK 465     SER C   781                                                      
    REMARK 465     GLY C   782                                                      
    REMARK 465     SER C   783                                                      
    REMARK 465     GLU C   784                                                      
    REMARK 465     ASN C   785                                                      
    REMARK 465     LEU C   786                                                      
    REMARK 465     TYR C   787                                                      
    REMARK 465     PHE C   788                                                      
    REMARK 465     GLN C   789                                                      
    REMARK 500                                                                      
    REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
    REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
    REMARK 500                                                                      
    REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
    REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
    REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
    REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
    REMARK 500                                                                      
    REMARK 500 STANDARD TABLE:                                                      
    REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
    REMARK 500                                                                      
    REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
    REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
    REMARK 500                                                                      
    REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
    REMARK 500      A V   1   P       A V   1   OP3    -0.127                       
    REMARK 500                                                                      
    REMARK 500 REMARK: NULL                                                         
    REMARK 500                                                                      
    REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
    REMARK 500 SUBTOPIC: TORSION ANGLES                                             
    REMARK 500                                                                      
    REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
    REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
    REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
    REMARK 500                                                                      
    REMARK 500 STANDARD TABLE:                                                      
    REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
    REMARK 500                                                                      
    REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
    REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
    REMARK 500                                                                      
    REMARK 500  M RES CSSEQI        PSI       PHI                                   
    REMARK 500    SER A  25       71.49     43.05                                   
    REMARK 500    ASN A 141       37.31   -150.61                                   
    REMARK 500    GLU A 159       46.86    -99.88                                   
    REMARK 500    MET A 285      -92.61   -122.82                                   
    REMARK 500    VAL A 428      -55.71   -132.76                                   
    REMARK 500    SER A 505       57.62   -144.63                                   
    REMARK 500    ARG A 508      -73.52    -80.48                                   
    REMARK 500    SER A 547      -81.74   -130.01                                   
    REMARK 500    GLU A 621       58.87     37.33                                   
    REMARK 500    ASP A 678       81.44   -160.34                                   
    REMARK 500    SER A 691      -89.25   -128.33                                   
    REMARK 500    ARG B 126     -156.40     60.23                                   
    REMARK 500    SER B 375       40.52    -85.15                                   
    REMARK 500    ILE B 376      133.13   -172.95                                   
    REMARK 500    LEU B 404       68.96   -155.62                                   
    REMARK 500    MET B 410      102.79    -50.42                                   
    REMARK 500    PHE B 413       64.29   -104.52                                   
    REMARK 500    LYS B 428      -38.78     69.01                                   
    REMARK 500    SER B 443     -127.48     54.63                                   
    REMARK 500    PHE B 491      116.70   -163.78                                   
    REMARK 500    VAL B 516      -63.63    -93.52                                   
    REMARK 500    SER B 664       46.82    -85.89                                   
    REMARK 500    LYS C 154      112.74   -162.52                                   
    REMARK 500                                                                      
    REMARK 500 REMARK: NULL                                                         
    DBREF  4WRT R    1    18  PDB    4WRT     4WRT             1     18             
    DBREF  4WRT V    1    14  PDB    4WRT     4WRT             1     14             
    DBREF  4WRT A    1   726  UNP    Q5V8Z9   Q5V8Z9_9INFB     1    726             
    DBREF  4WRT B    1   752  UNP    Q5V8Y6   Q5V8Y6_9INFB     1    752             
    DBREF  4WRT C    1   770  UNP    Q5V8X3   Q5V8X3_9INFB     1    770             
    SEQADV 4WRT GLY A  -13  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT SER A  -12  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A  -11  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A  -10  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A   -9  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A   -8  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A   -7  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A   -6  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A   -5  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT HIS A   -4  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLY A   -3  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT SER A   -2  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLY A   -1  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT SER A    0  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLY A  727  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT SER A  728  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLY A  729  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT SER A  730  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLY A  731  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLU A  732  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT ASN A  733  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT LEU A  734  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT TYR A  735  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT PHE A  736  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLN A  737  UNP  Q5V8Z9              EXPRESSION TAG                 
    SEQADV 4WRT GLY B   -8  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT SER B   -7  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY B   -6  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT SER B   -5  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY B   -4  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT SER B   -3  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY B   -2  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT SER B   -1  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY B    0  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY B  753  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT SER B  754  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY B  755  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT SER B  756  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY B  757  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLU B  758  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT ASN B  759  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT LEU B  760  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT TYR B  761  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT PHE B  762  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLN B  763  UNP  Q5V8Y6              EXPRESSION TAG                 
    SEQADV 4WRT GLY C   -8  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT SER C   -7  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLY C   -6  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT SER C   -5  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLY C   -4  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT SER C   -3  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLY C   -2  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT SER C   -1  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLY C    0  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLY C  771  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT TRP C  772  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT SER C  773  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT HIS C  774  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT PRO C  775  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLN C  776  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT PHE C  777  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLU C  778  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT LYS C  779  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLY C  780  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT SER C  781  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLY C  782  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT SER C  783  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLU C  784  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT ASN C  785  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT LEU C  786  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT TYR C  787  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT PHE C  788  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQADV 4WRT GLN C  789  UNP  Q5V8X3              EXPRESSION TAG                 
    SEQRES   1 R   18    U   A   U   A   C   C   U   C   U   G   C   U   U          
    SEQRES   2 R   18    C   U   G   C   U                                          
    SEQRES   1 V   14    A   G   U   A   G   U   A   A   C   A   A   G   A          
    SEQRES   2 V   14    G                                                          
    SEQRES   1 A  751  GLY SER HIS HIS HIS HIS HIS HIS HIS HIS GLY SER GLY          
    SEQRES   2 A  751  SER MET ASP THR PHE ILE THR ARG ASN PHE GLN THR THR          
    SEQRES   3 A  751  ILE ILE GLN LYS ALA LYS ASN THR MET ALA GLU PHE SER          
    SEQRES   4 A  751  GLU ASP PRO GLU LEU GLN PRO ALA MET LEU PHE ASN ILE          
    SEQRES   5 A  751  CYS VAL HIS LEU GLU VAL CYS TYR VAL ILE SER ASP MET          
    SEQRES   6 A  751  ASN PHE LEU ASP GLU GLU GLY LYS ALA TYR THR ALA LEU          
    SEQRES   7 A  751  GLU GLY GLN GLY LYS GLU GLN ASN LEU ARG PRO GLN TYR          
    SEQRES   8 A  751  GLU VAL ILE GLU GLY MET PRO ARG THR ILE ALA TRP MET          
    SEQRES   9 A  751  VAL GLN ARG SER LEU ALA GLN GLU HIS GLY ILE GLU THR          
    SEQRES  10 A  751  PRO LYS TYR LEU ALA ASP LEU PHE ASP TYR LYS THR LYS          
    SEQRES  11 A  751  ARG PHE ILE GLU VAL GLY ILE THR LYS GLY LEU ALA ASP          
    SEQRES  12 A  751  ASP TYR PHE TRP LYS LYS LYS GLU LYS LEU GLY ASN SER          
    SEQRES  13 A  751  MET GLU LEU MET ILE PHE SER TYR ASN GLN ASP TYR SER          
    SEQRES  14 A  751  LEU SER ASN GLU SER SER LEU ASP GLU GLU GLY LYS GLY          
    SEQRES  15 A  751  ARG VAL LEU SER ARG LEU THR GLU LEU GLN ALA GLU LEU          
    SEQRES  16 A  751  SER LEU LYS ASN LEU TRP GLN VAL LEU ILE GLY GLU GLU          
    SEQRES  17 A  751  ASP VAL GLU LYS GLY ILE ASP PHE LYS LEU GLY GLN THR          
    SEQRES  18 A  751  ILE SER ARG LEU ARG ASP ILE SER VAL PRO ALA GLY PHE          
    SEQRES  19 A  751  SER ASN PHE GLU GLY MET ARG SER TYR ILE ASP ASN ILE          
    SEQRES  20 A  751  ASP PRO LYS GLY ALA ILE GLU ARG ASN LEU ALA ARG MET          
    SEQRES  21 A  751  SER PRO LEU VAL SER VAL THR PRO LYS LYS LEU THR TRP          
    SEQRES  22 A  751  GLU ASP LEU ARG PRO ILE GLY PRO HIS ILE TYR ASN HIS          
    SEQRES  23 A  751  GLU LEU PRO GLU VAL PRO TYR ASN ALA PHE LEU LEU MET          
    SEQRES  24 A  751  SER ASP GLU LEU GLY LEU ALA ASN MET THR GLU GLY LYS          
    SEQRES  25 A  751  SER LYS LYS PRO LYS THR LEU ALA LYS GLU CYS LEU GLU          
    SEQRES  26 A  751  LYS TYR SER THR LEU ARG ASP GLN THR ASP PRO ILE LEU          
    SEQRES  27 A  751  ILE MET LYS SER GLU LYS ALA ASN GLU ASN PHE LEU TRP          
    SEQRES  28 A  751  LYS LEU TRP ARG ASP CYS VAL ASN THR ILE SER ASN GLU          
    SEQRES  29 A  751  GLU MET SER ASN GLU LEU GLN LYS THR ASN TYR ALA LYS          
    SEQRES  30 A  751  TRP ALA THR GLY ASP GLY LEU THR TYR GLN LYS ILE MET          
    SEQRES  31 A  751  LYS GLU VAL ALA ILE ASP ASP GLU THR MET CYS GLN GLU          
    SEQRES  32 A  751  GLU PRO LYS ILE PRO ASN LYS CYS ARG VAL ALA ALA TRP          
    SEQRES  33 A  751  VAL GLN THR GLU MET ASN LEU LEU SER THR LEU THR SER          
    SEQRES  34 A  751  LYS ARG ALA LEU ASP LEU PRO GLU ILE GLY PRO ASP VAL          
    SEQRES  35 A  751  ALA PRO VAL GLU HIS VAL GLY SER GLU ARG ARG LYS TYR          
    SEQRES  36 A  751  PHE VAL ASN GLU ILE ASN TYR CYS LYS ALA SER THR VAL          
    SEQRES  37 A  751  MET MET LYS TYR VAL LEU PHE HIS THR SER LEU LEU ASN          
    SEQRES  38 A  751  GLU SER ASN ALA SER MET GLY LYS TYR LYS VAL ILE PRO          
    SEQRES  39 A  751  ILE THR ASN ARG VAL VAL ASN GLU LYS GLY GLU SER PHE          
    SEQRES  40 A  751  ASP MET LEU TYR GLY LEU ALA VAL LYS GLY GLN SER HIS          
    SEQRES  41 A  751  LEU ARG GLY ASP THR ASP VAL VAL THR VAL VAL THR PHE          
    SEQRES  42 A  751  GLU PHE SER SER THR ASP PRO ARG VAL ASP SER GLY LYS          
    SEQRES  43 A  751  TRP PRO LYS TYR THR VAL PHE ARG ILE GLY SER LEU PHE          
    SEQRES  44 A  751  VAL SER GLY ARG GLU LYS SER VAL TYR LEU TYR CYS ARG          
    SEQRES  45 A  751  VAL ASN GLY THR ASN LYS ILE GLN MET LYS TRP GLY MET          
    SEQRES  46 A  751  GLU ALA ARG ARG CYS LEU LEU GLN SER MET GLN GLN MET          
    SEQRES  47 A  751  GLU ALA ILE VAL GLU GLN GLU SER SER ILE GLN GLY TYR          
    SEQRES  48 A  751  ASP MET THR LYS ALA CYS PHE LYS GLY ASP ARG VAL ASN          
    SEQRES  49 A  751  SER PRO LYS THR PHE SER ILE GLY THR GLN GLU GLY LYS          
    SEQRES  50 A  751  LEU VAL LYS GLY SER PHE GLY LYS ALA LEU ARG VAL ILE          
    SEQRES  51 A  751  PHE THR LYS CYS LEU MET HIS TYR VAL PHE GLY ASN ALA          
    SEQRES  52 A  751  GLN LEU GLU GLY PHE SER ALA GLU SER ARG ARG LEU LEU          
    SEQRES  53 A  751  LEU LEU ILE GLN ALA LEU LYS ASP ARG LYS GLY PRO TRP          
    SEQRES  54 A  751  VAL PHE ASP LEU GLU GLY MET TYR SER GLY ILE GLU GLU          
    SEQRES  55 A  751  CYS ILE SER ASN ASN PRO TRP VAL ILE GLN SER ALA TYR          
    SEQRES  56 A  751  TRP PHE ASN GLU TRP LEU GLY PHE GLU LYS GLU GLY SER          
    SEQRES  57 A  751  LYS VAL LEU GLU SER VAL ASP GLU ILE MET ASP GLU GLY          
    SEQRES  58 A  751  SER GLY SER GLY GLU ASN LEU TYR PHE GLN                      
    SEQRES   1 B  772  GLY SER GLY SER GLY SER GLY SER GLY MET ASN ILE ASN          
    SEQRES   2 B  772  PRO TYR PHE LEU PHE ILE ASP VAL PRO ILE GLN ALA ALA          
    SEQRES   3 B  772  ILE SER THR THR PHE PRO TYR THR GLY VAL PRO PRO TYR          
    SEQRES   4 B  772  SER HIS GLY THR GLY THR GLY TYR THR ILE ASP THR VAL          
    SEQRES   5 B  772  ILE ARG THR HIS GLU TYR SER ASN LYS GLY LYS GLN TYR          
    SEQRES   6 B  772  ILE SER ASP VAL THR GLY CYS THR MET VAL ASP PRO THR          
    SEQRES   7 B  772  ASN GLY PRO LEU PRO GLU ASP ASN GLU PRO SER ALA TYR          
    SEQRES   8 B  772  ALA GLN LEU ASP CYS VAL LEU GLU ALA LEU ASP ARG MET          
    SEQRES   9 B  772  ASP GLU GLU HIS PRO GLY LEU PHE GLN ALA ALA SER GLN          
    SEQRES  10 B  772  ASN ALA MET GLU THR LEU MET VAL THR THR VAL ASP LYS          
    SEQRES  11 B  772  LEU THR GLN GLY ARG GLN THR PHE ASP TRP THR VAL CYS          
    SEQRES  12 B  772  ARG ASN GLN PRO ALA ALA THR ALA LEU ASN THR THR ILE          
    SEQRES  13 B  772  THR SER PHE ARG LEU ASN ASP LEU ASN GLY ALA ASP LYS          
    SEQRES  14 B  772  GLY GLY LEU ILE PRO PHE CYS GLN ASP ILE ILE ASP SER          
    SEQRES  15 B  772  LEU ASP ARG PRO GLU MET THR PHE PHE SER VAL LYS ASN          
    SEQRES  16 B  772  ILE LYS LYS LYS LEU PRO ALA LYS ASN ARG LYS GLY PHE          
    SEQRES  17 B  772  LEU ILE LYS ARG ILE PRO MET LYS VAL LYS ASP LYS ILE          
    SEQRES  18 B  772  THR LYS VAL GLU TYR ILE LYS ARG ALA LEU SER LEU ASN          
    SEQRES  19 B  772  THR MET THR LYS ASP ALA GLU ARG GLY LYS LEU LYS ARG          
    SEQRES  20 B  772  ARG ALA ILE ALA THR ALA GLY ILE GLN ILE ARG GLY PHE          
    SEQRES  21 B  772  VAL LEU VAL VAL GLU ASN LEU ALA LYS ASN ILE CYS GLU          
    SEQRES  22 B  772  ASN LEU GLU GLN SER GLY LEU PRO VAL GLY GLY ASN GLU          
    SEQRES  23 B  772  LYS LYS ALA LYS LEU SER ASN ALA VAL ALA LYS MET LEU          
    SEQRES  24 B  772  SER ASN CYS PRO PRO GLY GLY ILE SER MET THR VAL THR          
    SEQRES  25 B  772  GLY ASP ASN THR LYS TRP ASN GLU CYS LEU ASN PRO ARG          
    SEQRES  26 B  772  ILE PHE LEU ALA MET THR GLU ARG ILE THR ARG ASP SER          
    SEQRES  27 B  772  PRO ILE TRP PHE ARG ASP PHE CYS SER ILE ALA PRO VAL          
    SEQRES  28 B  772  LEU PHE SER ASN LYS ILE ALA ARG LEU GLY LYS GLY PHE          
    SEQRES  29 B  772  MET ILE THR SER LYS THR LYS ARG LEU LYS ALA GLN ILE          
    SEQRES  30 B  772  PRO CYS PRO ASP LEU PHE SER ILE PRO LEU GLU ARG TYR          
    SEQRES  31 B  772  ASN GLU GLU THR ARG ALA LYS LEU LYS LYS LEU LYS PRO          
    SEQRES  32 B  772  PHE PHE ASN GLU GLU GLY THR ALA SER LEU SER PRO GLY          
    SEQRES  33 B  772  MET MET MET GLY MET PHE ASN MET LEU SER THR VAL LEU          
    SEQRES  34 B  772  GLY VAL ALA ALA LEU GLY ILE LYS ASN ILE GLY ASN LYS          
    SEQRES  35 B  772  GLU TYR LEU TRP ASP GLY LEU GLN SER SER ASP ASP PHE          
    SEQRES  36 B  772  ALA LEU PHE VAL ASN ALA LYS ASP GLU GLU THR CYS MET          
    SEQRES  37 B  772  GLU GLY ILE ASN ASP PHE TYR ARG THR CYS LYS LEU LEU          
    SEQRES  38 B  772  GLY ILE ASN MET SER LYS LYS LYS SER TYR CYS ASN GLU          
    SEQRES  39 B  772  THR GLY MET PHE GLU PHE THR SER MET PHE TYR ARG ASP          
    SEQRES  40 B  772  GLY PHE VAL SER ASN PHE ALA MET GLU LEU PRO SER PHE          
    SEQRES  41 B  772  GLY VAL ALA GLY VAL ASN GLU SER ALA ASP MET ALA ILE          
    SEQRES  42 B  772  GLY MET THR ILE ILE LYS ASN ASN MET ILE ASN ASN GLY          
    SEQRES  43 B  772  MET GLY PRO ALA THR ALA GLN THR ALA ILE GLN LEU PHE          
    SEQRES  44 B  772  ILE ALA ASP TYR ARG TYR THR TYR LYS CYS HIS ARG GLY          
    SEQRES  45 B  772  ASP SER LYS VAL GLU GLY LYS ARG MET LYS ILE ILE LYS          
    SEQRES  46 B  772  GLU LEU TRP GLU ASN THR LYS GLY ARG ASP GLY LEU LEU          
    SEQRES  47 B  772  VAL ALA ASP GLY GLY PRO ASN ILE TYR ASN LEU ARG ASN          
    SEQRES  48 B  772  LEU HIS ILE PRO GLU ILE VAL LEU LYS TYR ASN LEU MET          
    SEQRES  49 B  772  ASP PRO GLU TYR LYS GLY ARG LEU LEU HIS PRO GLN ASN          
    SEQRES  50 B  772  PRO PHE VAL GLY HIS LEU SER ILE GLU GLY ILE LYS GLU          
    SEQRES  51 B  772  ALA ASP ILE THR PRO ALA HIS GLY PRO VAL LYS LYS MET          
    SEQRES  52 B  772  ASP TYR ASP ALA VAL SER GLY THR HIS SER TRP ARG THR          
    SEQRES  53 B  772  LYS ARG ASN ARG SER ILE LEU ASN THR ASP GLN ARG ASN          
    SEQRES  54 B  772  MET ILE LEU GLU GLU GLN CYS TYR ALA LYS CYS CYS ASN          
    SEQRES  55 B  772  LEU PHE GLU ALA CYS PHE ASN SER ALA SER TYR ARG LYS          
    SEQRES  56 B  772  PRO VAL GLY GLN HIS SER MET LEU GLU ALA MET ALA HIS          
    SEQRES  57 B  772  ARG LEU ARG MET ASP ALA ARG LEU ASP TYR GLU SER GLY          
    SEQRES  58 B  772  ARG MET SER LYS ASP ASP PHE GLU LYS ALA MET ALA HIS          
    SEQRES  59 B  772  LEU GLY GLU ILE GLY TYR ILE GLY SER GLY SER GLY GLU          
    SEQRES  60 B  772  ASN LEU TYR PHE GLN                                          
    SEQRES   1 C  798  GLY SER GLY SER GLY SER GLY SER GLY MET THR LEU ALA          
    SEQRES   2 C  798  LYS ILE GLU LEU LEU LYS GLN LEU LEU ARG ASP ASN GLU          
    SEQRES   3 C  798  ALA LYS THR VAL LEU LYS GLN THR THR VAL ASP GLN TYR          
    SEQRES   4 C  798  ASN ILE ILE ARG LYS PHE ASN THR SER ARG ILE GLU LYS          
    SEQRES   5 C  798  ASN PRO SER LEU ARG MET LYS TRP ALA MET CYS SER ASN          
    SEQRES   6 C  798  PHE PRO LEU ALA LEU THR LYS GLY ASP MET ALA ASN ARG          
    SEQRES   7 C  798  ILE PRO LEU GLU TYR LYS GLY ILE GLN LEU LYS THR ASN          
    SEQRES   8 C  798  ALA GLU ASP ILE GLY THR LYS GLY GLN MET CYS SER ILE          
    SEQRES   9 C  798  ALA ALA VAL THR TRP TRP ASN THR TYR GLY PRO ILE GLY          
    SEQRES  10 C  798  ASP THR GLU GLY PHE GLU ARG VAL TYR GLU SER PHE PHE          
    SEQRES  11 C  798  LEU ARG LYS MET ARG LEU ASP ASN ALA THR TRP GLY ARG          
    SEQRES  12 C  798  ILE THR PHE GLY PRO VAL GLU ARG VAL ARG LYS ARG VAL          
    SEQRES  13 C  798  LEU LEU ASN PRO LEU THR LYS GLU MET PRO PRO ASP GLU          
    SEQRES  14 C  798  ALA SER ASN VAL ILE MET GLU ILE LEU PHE PRO LYS GLU          
    SEQRES  15 C  798  ALA GLY ILE PRO ARG GLU SER THR TRP ILE HIS ARG GLU          
    SEQRES  16 C  798  LEU ILE LYS GLU LYS ARG GLU LYS LEU LYS GLY THR MET          
    SEQRES  17 C  798  ILE THR PRO ILE VAL LEU ALA TYR MET LEU GLU ARG GLU          
    SEQRES  18 C  798  LEU VAL ALA ARG ARG ARG PHE LEU PRO VAL ALA GLY ALA          
    SEQRES  19 C  798  THR SER ALA GLU PHE ILE GLU MET LEU HIS CYS LEU GLN          
    SEQRES  20 C  798  GLY GLU ASN TRP ARG GLN ILE TYR HIS PRO GLY GLY ASN          
    SEQRES  21 C  798  LYS LEU THR GLU SER ARG SER GLN SER MET ILE VAL ALA          
    SEQRES  22 C  798  CYS ARG LYS ILE ILE ARG ARG SER ILE VAL ALA SER ASN          
    SEQRES  23 C  798  PRO LEU GLU LEU ALA VAL GLU ILE ALA ASN LYS THR VAL          
    SEQRES  24 C  798  ILE ASP THR GLU PRO LEU LYS SER CYS LEU ALA ALA ILE          
    SEQRES  25 C  798  ASP GLY GLY ASP VAL ALA CYS ASP ILE ILE ARG ALA ALA          
    SEQRES  26 C  798  LEU GLY LEU LYS ILE ARG GLN ARG GLN ARG PHE GLY ARG          
    SEQRES  27 C  798  LEU GLU LEU LYS ARG ILE SER GLY ARG GLY PHE LYS ASN          
    SEQRES  28 C  798  ASP GLU GLU ILE LEU ILE GLY ASN GLY THR ILE GLN LYS          
    SEQRES  29 C  798  ILE GLY ILE TRP ASP GLY GLU GLU GLU PHE HIS VAL ARG          
    SEQRES  30 C  798  CYS GLY GLU CYS ARG GLY ILE LEU LYS LYS SER LYS MET          
    SEQRES  31 C  798  LYS LEU GLU LYS LEU LEU ILE ASN SER ALA LYS LYS GLU          
    SEQRES  32 C  798  ASP MET ARG ASP LEU ILE ILE LEU CYS MET VAL PHE SER          
    SEQRES  33 C  798  GLN ASP THR ARG MET PHE GLN GLY VAL ARG GLY GLU ILE          
    SEQRES  34 C  798  ASN PHE LEU ASN ARG ALA GLY GLN LEU LEU SER PRO MET          
    SEQRES  35 C  798  TYR GLN LEU GLN ARG TYR PHE LEU ASN ARG SER ASN ASP          
    SEQRES  36 C  798  LEU PHE ASP GLN TRP GLY TYR GLU GLU SER PRO LYS ALA          
    SEQRES  37 C  798  SER GLU LEU HIS GLY ILE ASN GLU SER MET ASN ALA SER          
    SEQRES  38 C  798  ASP TYR THR LEU LYS GLY VAL VAL VAL THR ARG ASN VAL          
    SEQRES  39 C  798  ILE ASP ASP PHE SER SER THR GLU THR GLU LYS VAL SER          
    SEQRES  40 C  798  ILE THR LYS ASN LEU SER LEU ILE LYS ARG THR GLY GLU          
    SEQRES  41 C  798  VAL ILE MET GLY ALA ASN ASP VAL SER GLU LEU GLU SER          
    SEQRES  42 C  798  GLN ALA GLN LEU MET ILE THR TYR ASP THR PRO LYS MET          
    SEQRES  43 C  798  TRP GLU MET GLY THR THR LYS GLU LEU VAL GLN ASN THR          
    SEQRES  44 C  798  TYR GLN TRP VAL LEU LYS ASN LEU VAL THR LEU LYS ALA          
    SEQRES  45 C  798  GLN PHE LEU LEU GLY LYS GLU ASP MET PHE GLN TRP ASP          
    SEQRES  46 C  798  ALA PHE GLU ALA PHE GLU SER ILE ILE PRO GLN LYS MET          
    SEQRES  47 C  798  ALA GLY GLN TYR SER GLY PHE ALA ARG ALA VAL LEU LYS          
    SEQRES  48 C  798  GLN MET ARG ASP GLN GLU VAL MET LYS THR ASP GLN PHE          
    SEQRES  49 C  798  ILE LYS LEU LEU PRO PHE CYS PHE SER PRO PRO LYS LEU          
    SEQRES  50 C  798  ARG SER ASN GLY GLU PRO TYR GLN PHE LEU LYS LEU VAL          
    SEQRES  51 C  798  LEU LYS GLY GLY GLY GLU ASN PHE ILE GLU VAL ARG LYS          
    SEQRES  52 C  798  GLY SER PRO LEU PHE SER TYR ASN PRO GLN THR GLU VAL          
    SEQRES  53 C  798  LEU THR ILE CYS GLY ARG MET MET SER LEU LYS GLY LYS          
    SEQRES  54 C  798  ILE GLU ASP GLU GLU ARG ASN ARG SER MET GLY ASN ALA          
    SEQRES  55 C  798  VAL LEU ALA GLY PHE LEU VAL SER GLY LYS TYR ASP PRO          
    SEQRES  56 C  798  ASP LEU GLY ASP PHE LYS THR ILE GLU GLU LEU GLU LYS          
    SEQRES  57 C  798  LEU LYS PRO GLY GLU LYS ALA ASN ILE LEU LEU TYR GLN          
    SEQRES  58 C  798  GLY LYS PRO VAL LYS VAL VAL LYS ARG LYS ARG TYR SER          
    SEQRES  59 C  798  ALA LEU SER ASN ASP ILE SER GLN GLY ILE LYS ARG GLN          
    SEQRES  60 C  798  ARG MET THR VAL GLU SER MET GLY TRP ALA LEU SER GLY          
    SEQRES  61 C  798  TRP SER HIS PRO GLN PHE GLU LYS GLY SER GLY SER GLU          
    SEQRES  62 C  798  ASN LEU TYR PHE GLN                                          
    FORMUL   6  HOH   *809(H2 O)                                                    
    HELIX    1 AA1 SER A    0  PHE A    9  1                                  10    
    HELIX    2 AA2 GLN A   10  PHE A   24  1                                  15    
    HELIX    3 AA3 GLN A   31  MET A   51  1                                  21    
    HELIX    4 AA4 PRO A   84  HIS A   99  1                                  16    
    HELIX    5 AA5 LEU A  127  GLY A  140  1                                  14    
    HELIX    6 AA6 ASP A  163  LYS A  184  1                                  22    
    HELIX    7 AA7 LEU A  186  GLY A  192  1                                   7    
    HELIX    8 AA8 GLY A  205  ILE A  214  1                                  10    
    HELIX    9 AA9 ASN A  222  ILE A  233  1                                  12    
    HELIX   10 AB1 GLY A  237  MET A  246  1                                  10    
    HELIX   11 AB2 THR A  258  LEU A  262  5                                   5    
    HELIX   12 AB3 GLY A  266  ASN A  271  5                                   6    
    HELIX   13 AB4 LYS A  301  TYR A  313  1                                  13    
    HELIX   14 AB5 TYR A  313  ASP A  318  1                                   6    
    HELIX   15 AB6 ASN A  332  ASN A  349  1                                  18    
    HELIX   16 AB7 THR A  359  THR A  366  1                                   8    
    HELIX   17 AB8 MET A  376  ASP A  383  1                                   8    
    HELIX   18 AB9 ALA A  400  SER A  411  1                                  12    
    HELIX   19 AC1 ALA A  429  TYR A  448  1                                  20    
    HELIX   20 AC2 CYS A  449  SER A  472  1                                  24    
    HELIX   21 AC3 ASP A  529  TRP A  533  5                                   5    
    HELIX   22 AC4 LYS A  564  GLU A  572  1                                   9    
    HELIX   23 AC5 ARG A  575  GLY A  596  1                                  22    
    HELIX   24 AC6 MET A  599  LYS A  605  1                                   7    
    HELIX   25 AC7 PHE A  629  PHE A  646  1                                  18    
    HELIX   26 AC8 ASN A  648  ASP A  670  1                                  23    
    HELIX   27 AC9 ASP A  678  GLU A  688  1                                  11    
    HELIX   28 AD1 ASN A  693  VAL A  716  1                                  24    
    HELIX   29 AD2 PHE B    7  VAL B   12  1                                   6    
    HELIX   30 AD3 ILE B   14  PHE B   22  5                                   9    
    HELIX   31 AD4 GLY B   35  TYR B   49  1                                  15    
    HELIX   32 AD5 GLN B   84  HIS B   99  1                                  16    
    HELIX   33 AD6 GLY B  101  THR B  117  1                                  17    
    HELIX   34 AD7 THR B  118  GLY B  125  5                                   8    
    HELIX   35 AD8 PRO B  138  LEU B  152  1                                  15    
    HELIX   36 AD9 ASN B  156  GLY B  161  5                                   6    
    HELIX   37 AE1 GLY B  162  SER B  173  1                                  12    
    HELIX   38 AE2 LEU B  174  ARG B  176  5                                   3    
    HELIX   39 AE3 LYS B  214  LEU B  222  1                                   9    
    HELIX   40 AE4 GLY B  245  GLU B  264  1                                  20    
    HELIX   41 AE5 GLY B  274  ASN B  292  1                                  19    
    HELIX   42 AE6 LYS B  308  LEU B  313  1                                   6    
    HELIX   43 AE7 ASN B  314  THR B  326  1                                  13    
    HELIX   44 AE8 PRO B  330  ASN B  346  1                                  17    
    HELIX   45 AE9 PRO B  369  LEU B  373  5                                   5    
    HELIX   46 AF1 PRO B  377  TYR B  381  5                                   5    
    HELIX   47 AF2 ASN B  382  LYS B  393  1                                  12    
    HELIX   48 AF3 PHE B  413  GLY B  426  1                                  14    
    HELIX   49 AF4 ASP B  454  LEU B  471  1                                  18    
    HELIX   50 AF5 ASN B  503  GLY B  512  5                                  10    
    HELIX   51 AF6 ASN B  517  ASN B  536  1                                  20    
    HELIX   52 AF7 GLY B  539  TYR B  558  1                                  20    
    HELIX   53 AF8 GLY B  569  THR B  582  1                                  14    
    HELIX   54 AF9 GLY B  584  LEU B  588  5                                   5    
    HELIX   55 AG1 LEU B  589  GLY B  593  5                                   5    
    HELIX   56 AG2 ASN B  599  LEU B  603  5                                   5    
    HELIX   57 AG3 PRO B  606  TYR B  612  1                                   7    
    HELIX   58 AG4 ASN B  613  MET B  615  5                                   3    
    HELIX   59 AG5 ASP B  616  LEU B  624  1                                   9    
    HELIX   60 AG6 SER B  635  LYS B  640  1                                   6    
    HELIX   61 AG7 ARG B  671  THR B  676  5                                   6    
    HELIX   62 AG8 ARG B  679  PHE B  699  1                                  21    
    HELIX   63 AG9 SER B  712  SER B  731  1                                  20    
    HELIX   64 AH1 SER B  735  ILE B  749  1                                  15    
    HELIX   65 AH2 MET C    1  ASP C   15  1                                  15    
    HELIX   66 AH3 ASP C   15  THR C   25  1                                  11    
    HELIX   67 AH4 THR C   26  ARG C   34  1                                   9    
    HELIX   68 AH5 ASN C   44  MET C   53  1                                  10    
    HELIX   69 AH6 GLY C   64  ARG C   69  1                                   6    
    HELIX   70 AH7 SER C   94  GLY C  105  1                                  12    
    HELIX   71 AH8 GLY C  112  TYR C  117  1                                   6    
    HELIX   72 AH9 TYR C  117  ASN C  129  1                                  13    
    HELIX   73 AI1 PRO C  157  PHE C  170  1                                  14    
    HELIX   74 AI2 PRO C  171  ALA C  174  5                                   4    
    HELIX   75 AI3 ARG C  178  HIS C  184  1                                   7    
    HELIX   76 AI4 HIS C  184  LEU C  195  1                                  12    
    HELIX   77 AI5 THR C  201  GLU C  212  1                                  12    
    HELIX   78 AI6 SER C  227  GLU C  232  1                                   6    
    HELIX   79 AI7 MET C  233  GLN C  238  1                                   6    
    SHEET    1 AA1 2 ASN A  52  LEU A  54  0                                        
    SHEET    2 AA1 2 ALA A  60  THR A  62 -1  O  TYR A  61   N  PHE A  53           
    SHEET    1 AA2 5 TYR A  77  VAL A  79  0                                        
    SHEET    2 AA2 5 LEU A 110  ASP A 112 -1  O  PHE A 111   N  GLU A  78           
    SHEET    3 AA2 5 ARG A 117  THR A 124 -1  O  ILE A 119   N  LEU A 110           
    SHEET    4 AA2 5 GLU A 144  SER A 149  1  O  GLU A 144   N  PHE A 118           
    SHEET    5 AA2 5 TYR A 154  LEU A 156 -1  O  SER A 155   N  ILE A 147           
    SHEET    1 AA3 6 LYS A 255  LYS A 256  0                                        
    SHEET    2 AA3 6 TRP A 675  VAL A 676 -1  O  VAL A 676   N  LYS A 255           
    SHEET    3 AA3 6 TYR B 482  GLU B 485 -1  O  CYS B 483   N  TRP A 675           
    SHEET    4 AA3 6 ILE B 298  THR B 303 -1  N  THR B 301   O  ASN B 484           
    SHEET    5 AA3 6 ASP B 445  ALA B 452 -1  O  LEU B 448   N  VAL B 302           
    SHEET    6 AA3 6 TYR B 435  SER B 442 -1  N  ASP B 438   O  PHE B 449           
    SHEET    1 AA4 6 LEU A 291  THR A 295  0                                        
    SHEET    2 AA4 6 SER A 492  LYS A 502 -1  O  ASP A 494   N  ASN A 293           
    SHEET    3 AA4 6 VAL A 513  SER A 522 -1  O  PHE A 521   N  TYR A 497           
    SHEET    4 AA4 6 GLU A 550  ASN A 563 -1  O  TYR A 554   N  SER A 522           
    SHEET    5 AA4 6 THR A 537  PHE A 545 -1  N  THR A 537   O  CYS A 557           
    SHEET    6 AA4 6 ILE A 323  MET A 326 -1  N  ILE A 325   O  VAL A 538           
    SHEET    1 AA5 4 LEU A 291  THR A 295  0                                        
    SHEET    2 AA5 4 SER A 492  LYS A 502 -1  O  ASP A 494   N  ASN A 293           
    SHEET    3 AA5 4 TYR A 476  VAL A 486 -1  N  LYS A 477   O  LYS A 502           
    SHEET    4 AA5 4 GLU A 355  LEU A 356 -1  N  LEU A 356   O  TYR A 476           
    SHEET    1 AA6 3 GLN A 373  LYS A 374  0                                        
    SHEET    2 AA6 3 LEU B 364  ILE B 368  1  O  LYS B 365   N  GLN A 373           
    SHEET    3 AA6 3 PHE B 355  SER B 359 -1  N  PHE B 355   O  ILE B 368           
    SHEET    1 AA7 3 LYS A 623  SER A 628  0                                        
    SHEET    2 AA7 3 THR A 614  GLN A 620 -1  N  ILE A 617   O  VAL A 625           
    SHEET    3 AA7 3 ASN B   2  ILE B   3 -1  O  ASN B   2   N  THR A 619           
    SHEET    1 AA8 2 LYS B  54  ILE B  57  0                                        
    SHEET    2 AA8 2 THR B  64  ASP B  67 -1  O  MET B  65   N  TYR B  56           
    SHEET    1 AA9 2 THR B 128  ASP B 130  0                                        
    SHEET    2 AA9 2 ARG B 135  GLN B 137 -1  O  GLN B 137   N  THR B 128           
    SHEET    1 AB1 2 GLU B 178  PRO B 192  0                                        
    SHEET    2 AB1 2 PHE B 199  THR B 213 -1  O  ASP B 210   N  PHE B 181           
    SHEET    1 AB2 5 ALA B 240  ALA B 244  0                                        
    SHEET    2 AB2 5 SER B 223  THR B 228 -1  N  MET B 227   O  ILE B 241           
    SHEET    3 AB2 5 ILE B 348  ARG B 350  1  O  ARG B 350   N  LEU B 224           
    SHEET    4 AB2 5 THR B 401  SER B 403 -1  O  ALA B 402   N  ALA B 349           
    SHEET    5 AB2 5 PHE B 396  ASN B 397 -1  N  ASN B 397   O  THR B 401           
    SHEET    1 AB3 2 MET B 488  PHE B 491  0                                        
    SHEET    2 AB3 2 MET B 494  ARG B 497 -1  O  TYR B 496   N  PHE B 489           
    SHEET    1 AB4 2 GLU B 641  ALA B 642  0                                        
    SHEET    2 AB4 2 MET B 654  ASP B 655 -1  O  MET B 654   N  ALA B 642           
    SHEET    1 AB5 3 LEU C  59  THR C  62  0                                        
    SHEET    2 AB5 3 LYS C  89  CYS C  93 -1  O  CYS C  93   N  LEU C  59           
    SHEET    3 AB5 3 LYS C  80  GLU C  84 -1  N  THR C  81   O  MET C  92           
    SHEET    1 AB6 2 GLU C  73  TYR C  74  0                                        
    SHEET    2 AB6 2 ILE C  77  GLN C  78 -1  O  ILE C  77   N  TYR C  74           
    SHEET    1 AB7 2 THR C 131  PHE C 137  0                                        
    SHEET    2 AB7 2 TRP C 242  HIS C 247 -1  O  HIS C 247   N  THR C 131           
    SHEET    1 AB8 2 VAL C 143  LEU C 148  0                                        
    SHEET    2 AB8 2 ARG C 217  VAL C 222 -1  O  ARG C 218   N  VAL C 147           
    CISPEP   1 LEU B  271    PRO B  272          0         2.51                     
    CRYST1  207.370  207.370  345.690  90.00  90.00 120.00 P 63 2 2     12          
    ORIGX1      1.000000  0.000000  0.000000        0.00000                         
    ORIGX2      0.000000  1.000000  0.000000        0.00000                         
    ORIGX3      0.000000  0.000000  1.000000        0.00000                         
    SCALE1      0.004822  0.002784  0.000000        0.00000                         
    SCALE2      0.000000  0.005568  0.000000        0.00000                         
    SCALE3      0.000000  0.000000  0.002893        0.00000                         

    3D molecular view of vibration

    Displacement vectors
    Display
    Animation
    Display

    Still image of displacement vectors and GIF animation


    Mode 1

    Time-average properties and properties of the 10 lowest-frequency modes

    Fluctuation of atoms:
    Time average and for the 3 lowest-frequency modes.
    Fluctuation of dihedral angles:
    Time average and for the 3 lowest-frequency modes.
    Fluctuation of atomsFluctuation of dihedral angles

    Correlations between fluctuations of atoms

    Mode 1
    Correlations between fluctuations of atoms - Mode 1
    Time Average
    Time Average
    Distance map
    Distance map

    Calculation note

    PDB file name : pdb4wrt.ent

    Chains and HETATMs selected: 
      ATOM        R
      ATOM        V
      ATOM        A
      ATOM        B
      ATOM        C

    The following atoms are removed from PDB data on concern that they may have 
    abnormally large fluctuations, because they interact with few atoms.
      ATOM   1404  CG  LYS A 105      66.323 -31.748 -51.510  1.00 64.42           C  
      ATOM   1405  CD  LYS A 105      64.896 -31.344 -51.190  1.00 76.76           C  
      ATOM   1406  CE  LYS A 105      64.253 -30.681 -52.404  1.00 89.78           C  
      ATOM   1407  NZ  LYS A 105      62.859 -30.218 -52.162  1.00 82.06           N  
      ATOM   1654  CG  LYS A 134      60.678 -45.001 -39.143  1.00 86.04           C  
      ATOM   1655  CD  LYS A 134      61.395 -44.841 -37.810  1.00 88.14           C  
      ATOM   1656  CE  LYS A 134      60.445 -45.049 -36.640  1.00 91.59           C  
      ATOM   1657  NZ  LYS A 134      61.120 -44.864 -35.325  1.00 83.47           N  
      ATOM   2468  CG  LYS A 236      74.210   1.379  -4.645  1.00 47.81           C  
      ATOM   2469  CD  LYS A 236      72.738   1.027  -4.791  1.00 83.48           C  
      ATOM   2470  CE  LYS A 236      71.893   2.267  -5.052  1.00 85.81           C  
      ATOM   2471  NZ  LYS A 236      70.457   1.934  -5.275  1.00 60.30           N  
      ATOM   3598  CG  MET A 376     130.887  -4.078 -37.991  1.00 78.14           C  
      ATOM   3599  SD  MET A 376     130.654  -5.854 -38.183  1.00118.89           S  
      ATOM   3600  CE  MET A 376     130.512  -5.972 -39.967  1.00 88.15           C  
      ATOM   3724  CG  LYS A 392     120.997  20.606 -35.028  1.00 65.69           C  
      ATOM   3725  CD  LYS A 392     121.286  21.139 -36.422  1.00 64.59           C  
      ATOM   3726  CE  LYS A 392     122.750  21.499 -36.597  1.00 83.77           C  
      ATOM   3727  NZ  LYS A 392     123.000  22.152 -37.913  1.00 85.53           N  
      ATOM   4960  CG  ARG A 549     112.874  54.124 -66.973  1.00107.02           C  
      ATOM   4961  CD  ARG A 549     112.693  54.849 -65.652  1.00107.29           C  
      ATOM   4962  NE  ARG A 549     112.055  56.150 -65.836  1.00128.93           N  
      ATOM   4963  CZ  ARG A 549     111.768  56.995 -64.850  1.00122.39           C  
      ATOM   4964  NH1 ARG A 549     112.063  56.685 -63.594  1.00113.63           N  
      ATOM   4965  NH2 ARG A 549     111.185  58.154 -65.121  1.00106.97           N  
      ATOM   5437  CG  ARG A 608      67.981  45.951 -60.700  1.00 78.04           C  
      ATOM   5438  CD  ARG A 608      66.879  45.264 -61.498  1.00 77.81           C  
      ATOM   5439  NE  ARG A 608      67.316  44.921 -62.849  1.00 75.59           N  
      ATOM   5440  CZ  ARG A 608      67.215  45.728 -63.901  1.00 71.20           C  
      ATOM   5441  NH1 ARG A 608      66.686  46.938 -63.768  1.00 61.16           N  
      ATOM   5442  NH2 ARG A 608      67.643  45.325 -65.090  1.00 63.33           N  
      ATOM   7810  CG  LYS B 194     138.182 -18.706 -59.086  1.00119.02           C  
      ATOM   7811  CD  LYS B 194     137.341 -18.229 -60.257  1.00118.93           C  
      ATOM   7812  CE  LYS B 194     136.188 -19.183 -60.527  1.00114.09           C  
      ATOM   7813  NZ  LYS B 194     135.342 -18.744 -61.671  1.00112.46           N  
      ATOM   7827  CG  ARG B 196     147.055 -13.572 -54.564  1.00128.74           C  
      ATOM   7828  CD  ARG B 196     147.783 -12.418 -53.893  1.00125.98           C  
      ATOM   7829  NE  ARG B 196     148.410 -11.520 -54.858  1.00133.87           N  
      ATOM   7830  CZ  ARG B 196     149.033 -10.391 -54.534  1.00131.86           C  
      ATOM   7831  NH1 ARG B 196     149.115 -10.015 -53.264  1.00124.91           N  
      ATOM   7832  NH2 ARG B 196     149.574  -9.635 -55.479  1.00125.93           N  
      ATOM   8540  CG  LYS B 288      66.289  13.023 -27.153  1.00 82.89           C  
      ATOM   8541  CD  LYS B 288      66.502  11.536 -27.361  1.00 98.36           C  
      ATOM   8542  CE  LYS B 288      65.409  10.948 -28.238  1.00 90.31           C  
      ATOM   8543  NZ  LYS B 288      65.720   9.561 -28.681  1.00 90.22           N  
      ATOM   9104  CG  LYS B 360     130.975  16.587 -36.656  1.00 65.68           C  
      ATOM   9105  CD  LYS B 360     130.540  17.872 -35.968  1.00 83.30           C  
      ATOM   9106  CE  LYS B 360     130.848  19.090 -36.828  1.00 83.85           C  
      ATOM   9107  NZ  LYS B 360     130.526  20.369 -36.135  1.00 66.69           N  
      ATOM  10860  CG  LYS B 583      86.301   6.439 -86.968  1.00 60.56           C  
      ATOM  10861  CD  LYS B 583      85.817   6.183 -88.383  1.00 67.32           C  
      ATOM  10862  CE  LYS B 583      84.845   7.274 -88.815  1.00 69.31           C  
      ATOM  10863  NZ  LYS B 583      84.268   7.047 -90.169  1.00 78.46           N  
      ATOM  11666  CG  LYS B 690     103.903 -23.759 -58.413  1.00 60.05           C  
      ATOM  11667  CD  LYS B 690     104.939 -24.765 -58.896  1.00 74.27           C  
      ATOM  11668  CE  LYS B 690     105.886 -24.150 -59.920  1.00 97.80           C  
      ATOM  11669  NZ  LYS B 690     106.521 -25.173 -60.800  1.00 78.62           N  
      ATOM  12077  CG  LYS B 741      97.869 -42.659 -70.498  1.00 96.54           C  
      ATOM  12078  CD  LYS B 741      98.224 -41.766 -71.674  1.00 94.26           C  
      ATOM  12079  CE  LYS B 741      99.205 -42.448 -72.613  1.00 84.71           C  
      ATOM  12080  NZ  LYS B 741      99.241 -41.799 -73.953  1.00 91.30           N  
      ATOM  12148  CG  MET C   1      91.196 -27.596 -69.694  1.00 82.49           C  
      ATOM  12149  SD  MET C   1      92.882 -27.286 -70.261  1.00128.28           S  
      ATOM  12150  CE  MET C   1      93.624 -26.570 -68.792  1.00 80.80           C  
      ATOM  12496  CG  LYS C  43     101.083   1.660 -43.828  1.00 51.73           C  
      ATOM  12497  CD  LYS C  43     102.173   1.462 -42.787  1.00 54.82           C  
      ATOM  12498  CE  LYS C  43     101.666   0.615 -41.625  1.00 83.69           C  
      ATOM  12499  NZ  LYS C  43     102.765   0.004 -40.824  1.00 78.93           N  
      ATOM  13223  CG  ARG C 134      62.575  25.084 -64.199  1.00 73.06           C  
      ATOM  13224  CD  ARG C 134      61.627  26.121 -63.623  1.00 81.37           C  
      ATOM  13225  NE  ARG C 134      61.211  27.126 -64.596  1.00101.26           N  
      ATOM  13226  CZ  ARG C 134      60.337  28.095 -64.337  1.00117.04           C  
      ATOM  13227  NH1 ARG C 134      59.779  28.186 -63.136  1.00114.73           N  
      ATOM  13228  NH2 ARG C 134      60.015  28.972 -65.277  1.00105.65           N  
      ATOM  13738  CG  LYS C 196      75.867 -21.040 -72.518  1.00 74.06           C  
      ATOM  13739  CD  LYS C 196      74.657 -21.746 -73.107  1.00 75.54           C  
      ATOM  13740  CE  LYS C 196      74.970 -23.210 -73.393  1.00 80.50           C  
      ATOM  13741  NZ  LYS C 196      73.794 -23.955 -73.921  1.00 85.08           N  

    = Normal mode analysis calculation =

    No. of modes used in the calculation : All modes.

    Parameters of potential energies:
      1-4 and 1-5 non-bonded interactions: E(d) = A*exp(-d(PDB)**2/B**2)(d-d(PDB))**2.
      Loop-closing potential:              E(d) = A*(d-d(PDB))**2.
        for a disulfide bond and one of the bonds in the DNA and RNA sugar ring.
      where d and d(PDB) are distances between atoms in calculation and in PDB data, 
      respectively.

      Interaction type   A       B      Cutoff distance (A)
        1-4             1.00    5.00      100.00
        1-5             1.00    5.00      100.00
        Loop-closing  100.00

    Temperature adjustment by magnitude of fluctuation:
      Set to mean displacements of atoms       0.500 A

    Animation
      No. of frames: 11
      Mean displacements (A):    0.50

    Displacement vector
      Mean length of vectors (A):    3.00