4WOI

4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	212.100, 435.240, 614.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.000 - 3.000
Rwork	0.174
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IN-HOUSE STRUCTURE CONTAINING ALL RIBOSOMAL PROTEINS IN ONE CHAIN AND RRNA IN ANOTHER
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000		3.000
High resolution limit [Å]	2.900	70.000	2.900
Rmerge	0.163		1.138
Rmeas	0.178		1.592
Total number of observations	4371132
Number of reflections	914752		4989
<I/σ(I)>	6.29		0.36
Completeness [%]	73.7		4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD, KCl, KSCN
1	MICROBATCH	6.5	291	PEG8k, MPD, KCl, KSCN