4WJG
Structure of T. brucei haptoglobin-hemoglobin receptor binding to human haptoglobin-hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 143.230, 140.950, 267.180 |
| Unit cell angles | 90.00, 98.54, 90.00 |
Refinement procedure
| Resolution | 29.000 - 3.100 |
| R-factor | 0.2553 |
| Rwork | 0.255 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 184077 | |
| <I/σ(I)> | 7.2 | 1.44 |
| Completeness [%] | 96.5 | 95.3 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 15% Polyethylene glycol (PEG) 1500, 0.1M Bis-Tris |
