4WIN
Crystal structure of the GATase domain from Plasmodium falciparum GMP synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93928 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.820, 74.210, 91.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.972 - 2.600 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpm |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.551 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.378 | |
| Number of reflections | 15347 | |
| <I/σ(I)> | 22.4 | 4.67 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 292 | 0.2 M Lithium Nitrate and 20% (w/v) PEG3350 |
