4WEF
Structure of the Hemagglutinin-neuraminidase from Human parainfluenza virus type III: complex with difluorosialic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 218.668, 218.668, 109.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.460 - 2.500 |
| R-factor | 0.19282 |
| Rwork | 0.189 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v3c |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 188.980 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.292 | 0.845 |
| Number of reflections | 50876 | |
| <I/σ(I)> | 7.5 | 1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Hepes, 1.0M (NH4)2SO4, 1.0M Li2SO4 |
