4W9M

AMPPNP bound Rad50 in complex with dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	49.713, 96.386, 105.926
Unit cell angles	90.01, 89.22, 81.67

Refinement procedure

Resolution	47.685 - 2.700
R-factor	0.2176
Rwork	0.215
R-free	0.27040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.337
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	51778
<I/σ(I)>	9.26
Completeness [%]	93.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	150 mM D-maleic acid pH 6.5, 21% (v/v) PEG3350