4W7S

Crystal structure of the yeast DEAD-box splicing factor Prp28 at 2.54 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-26
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	114.719, 114.719, 156.186
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.843 - 2.542
R-factor	0.1688
Rwork	0.166
R-free	0.21930
RMSD bond length	0.009
RMSD bond angle	1.227
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	57.000
High resolution limit [Å]	2.540
Number of reflections	39724
<I/σ(I)>	16.7
Completeness [%]	99.8
Redundancy	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	277	30% (v/v) PEG 400, 0.2 M MgCl2, 0.1 M Hepes-NaOH, pH 7.2-7.8, 0.1 M glycine