4W5N
The Crystal Structure of Human Argonaute2 Bound to a Defined Guide RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.117, 108.870, 68.071 |
Unit cell angles | 90.00, 106.93, 90.00 |
Refinement procedure
Resolution | 38.980 - 2.900 |
R-factor | 0.2192 |
Rwork | 0.217 |
R-free | 0.25330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ola |
RMSD bond length | 0.010 |
RMSD bond angle | 1.035 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.980 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.070 | |
Number of reflections | 19381 | |
<I/σ(I)> | 15.6 | 2.6 |
Completeness [%] | 98.8 | 98.8 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | PEG, Tris, Isopropanol, Phenol, Magnesium |