4W4Z

Structure of the EphA4 LBD in complex with peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-07
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	37.220, 127.190, 84.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.140 - 2.410
R-factor	0.1767
Rwork	0.173
R-free	0.24060
RMSD bond length	0.010
RMSD bond angle	1.419
Data reduction software	MOSFLM
Data scaling software	Aimless (0.2.1)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.830	50.830	2.510
High resolution limit [Å]	2.410	8.690	2.410
Rmerge	0.067	0.042	0.226
Rpim	0.041	0.025	0.138
Total number of observations	99518	2456	9181
Number of reflections	27979
<I/σ(I)>	13.1	20.6	4.8
Completeness [%]	92.3	98.1	80.6
Redundancy	3.6	3.7	3.3
CC(1/2)	0.996	0.997	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2M MgCl2, 0.1M Tris pH8.5, 25% PEG3350, 3% 1,6-hexanediol