4W4U
Structure of yeast SAGA DUBm with Sgf73 Y57A mutant at 2.8 angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.034 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.739, 67.273, 137.145 |
Unit cell angles | 90.00, 106.84, 90.00 |
Refinement procedure
Resolution | 47.020 - 2.800 |
R-factor | 0.18496 |
Rwork | 0.182 |
R-free | 0.24067 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mhs |
RMSD bond length | 0.011 |
RMSD bond angle | 1.371 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.020 |
High resolution limit [Å] | 2.800 |
Number of reflections | 34989 |
<I/σ(I)> | 23.2 |
Completeness [%] | 99.8 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM Bis Tris,18% PEG3350, 100mM Ammonium Sulfate |