4W4O
High-resolution crystal structure of Fc bound to its human receptor Fc-gamma-RI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.980, 126.500, 71.610 |
Unit cell angles | 90.00, 118.95, 90.00 |
Refinement procedure
Resolution | 39.700 - 1.800 |
R-factor | 0.17497 |
Rwork | 0.173 |
R-free | 0.21501 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1L6X and 3RJD |
RMSD bond length | 0.020 |
RMSD bond angle | 2.134 |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.700 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.074 | 0.870 |
Number of reflections | 95656 | |
<I/σ(I)> | 14.9 | 2.3 |
Completeness [%] | 98.6 | 97.4 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M sodium acetate, 0.1 M zinc acetate, 4% 1,4-butanediol, 12% PEG 4,000 |