4V8A
The structure of thermorubin in complex with the 70S ribosome from Thermus thermophilus.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.370, 445.460, 619.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.290 - 3.200 |
Rwork | 0.228 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.164 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.240 | 2.980 |
High resolution limit [Å] | 2.900 | 3.150 | 2.900 |
Rmerge | 0.717 | 2.650 | |
Number of reflections | 1262956 | ||
<I/σ(I)> | 1.73 | 0.65 | |
Completeness [%] | 99.9 | 100 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 2.9% PEG 20K, 9% MPD, 0.1M Tris-HCl pH 7.6, 175 mM L-Arginine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |