4V7E
Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome
This is a non-PDB format compatible entry.
Replaces: 3IZ5Replaces: 3IZ6Replaces: 3IZ7Replaces: 3IZ9Replaces: 3IZREM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | Blot for 10 seconds before plunging, use 2 layers of filter paper |
EM Data Collection
Microscope | FEI TECNAI F30 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.26 |
Detector | KODAK SO-163 FILM |
Number of Images | 1374 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 5.5 |
Software | SPIDER |
Symmetry | C1 |