4V7E

Model of the small subunit RNA based on a 5.5 A cryo-EM map of Triticum aestivum translating 80S ribosome

This is a non-PDB format compatible entry.

Replaces:  3IZ5Replaces:  3IZ6Replaces:  3IZ7Replaces:  3IZ9Replaces:  3IZR

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I
Method	Blot for 10 seconds before plunging, use 2 layers of filter paper

EM Data Collection

Microscope	FEI TECNAI F30
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.26
Detector	KODAK SO-163 FILM
Number of Images	1374

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	5.5
Software	SPIDER
Symmetry	C1