4V2G
Tetracycline repressor TetR(D) bound to chlortetracycline and iso- chlortetracycline
Replaces: 1BJYExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.249, 69.249, 182.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.740 - 2.710 |
| R-factor | 0.18203 |
| Rwork | 0.175 |
| R-free | 0.25156 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tct |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.622 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.230 |
| Number of reflections | 11449 | |
| <I/σ(I)> | 13 | 5 |
| Completeness [%] | 99.0 | 96 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PH 8.0 |
