4V0U
The crystal structure of ternary PP1G-PPP1R15B and G-actin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-18 |
| Detector | PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.910, 149.930, 318.720 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 82.790 - 7.880 |
| R-factor | 0.3718 |
| Rwork | 0.370 |
| R-free | 0.39976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BIY AND STRUCTURE OF MOUSE PP1G IN COMPLEX WITH TRUNCATED HUMAN PPP1R15B INCLUDING 631-660 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CCP4I |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.790 | 8.080 |
| High resolution limit [Å] | 7.880 | 7.880 |
| Rmerge | 0.140 | 0.680 |
| Number of reflections | 5111 | |
| <I/σ(I)> | 7.2 | 1.6 |
| Completeness [%] | 98.0 | 99.2 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M CACL2, 0.1 M HEPES, PH7.0, 20% PEG6000 |
