4UXD
2-keto 3-deoxygluconate aldolase from Picrophilus torridus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-26 |
Detector | SATURN 944PLUS |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 77.954, 100.159, 154.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.580 - 2.500 |
R-factor | 0.1792 |
Rwork | 0.175 |
R-free | 0.25950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xky |
RMSD bond length | 0.008 |
RMSD bond angle | 1.179 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.580 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.110 | 0.330 |
Number of reflections | 36955 | |
<I/σ(I)> | 13.25 | 2.65 |
Completeness [%] | 86.6 | 83.4 |
Redundancy | 3.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN AT 4.2MG/ML MIXED 1:1 WITH (0.085 M HEPES SODIUM BUFFER PH 7.5, 8.5% V/V 2-PROPANOL, 17% W/V PEG 4000, 15% V/V GLYCEROL (ANHYDROUS)) |