4UWB
Fibroblast growth factor receptor 1 kinase in complex with JK-P5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-18 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.623, 57.323, 65.628 |
| Unit cell angles | 90.00, 107.58, 90.00 |
Refinement procedure
| Resolution | 99.440 - 2.310 |
| R-factor | 0.23108 |
| Rwork | 0.229 |
| R-free | 0.27172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INTERNAL FGFR1 MODEL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.499 |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.440 | 2.310 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 29915 | |
| <I/σ(I)> | 12.8 | 3.1 |
| Completeness [%] | 96.9 | 94.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG8000, 20% ETHLYENE GLYCOL, 0.2M AMMONIUM SULPHATE, 0.1M PCTP PH 6.75 |
