4UTB
Crystal structure of dengue 2 virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.779, 181.850, 204.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.850 |
R-factor | 0.2311 |
Rwork | 0.230 |
R-free | 0.25670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h0t |
RMSD bond length | 0.008 |
RMSD bond angle | 1.180 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.220 |
High resolution limit [Å] | 3.850 | 3.850 |
Rmerge | 0.270 | 0.920 |
Number of reflections | 21168 | |
<I/σ(I)> | 5.8 | 1.4 |
Completeness [%] | 98.3 | 98.3 |
Redundancy | 4.83 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100MM HEPES PH 7.5 10% (W/V) PEG 6,000 5% (V/V) MPD |