4USG

Crystal structure of PC4 W89Y mutant complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	287
Detector technology	CCD
Collection date	2014-06-02
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	67.660, 67.660, 120.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.843 - 1.973
R-factor	0.1879
Rwork	0.185
R-free	0.21200
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.192
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.800
High resolution limit [Å]	1.970
Rmerge	0.010
Number of reflections	20387
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1