4URT

The crystal structure of a fragment of netrin-1 in complex with FN5- FN6 of DCC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-14
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	84.650, 87.410, 155.317
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.659 - 3.100
R-factor	0.2314
Rwork	0.230
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4aqt
RMSD bond length	0.021
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.150	0.820
Number of reflections	20291
<I/σ(I)>	10.8	1.8
Completeness [%]	99.3	96
Redundancy	8.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1M MES PH 6.0, 0.15M AMMONIUM SULFATE AND 15% (W/V) PEG 4000