4UIS
The cryoEM structure of human gamma-Secretase complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 1.4 |
| Detector | DIRECT ELECTRON DE-12 (4k x 3k) |
| Number of Images | 2000 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.4 |
| Software | UCSF Chimera,RELION |
| Symmetry | C1 |
