4UI0
Crystal structure of the human RGMB-BMP2 complex, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.021, 85.021, 115.182 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 2.800 |
| R-factor | 0.2344 |
| Rwork | 0.233 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.590 |
| Number of reflections | 13647 | |
| <I/σ(I)> | 14.7 | 2.7 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 9.6 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.08 M CITRIC ACID PH 4.0, 15% (V/V) MPD |
