4UF6
UCH-L5 in complex with ubiquitin-propargyl bound to an activating fragment of INO80G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.084, 137.788, 98.916 |
| Unit cell angles | 90.00, 102.54, 90.00 |
Refinement procedure
| Resolution | 100.990 - 3.690 |
| R-factor | 0.23956 |
| Rwork | 0.238 |
| R-free | 0.26907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF THE UCH-L5-UBIQUITIN FROM THE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.148 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 3.980 |
| High resolution limit [Å] | 3.690 | 3.690 |
| Rmerge | 0.200 | 0.660 |
| Number of reflections | 21090 | |
| <I/σ(I)> | 5.8 | 1.8 |
| Completeness [%] | 97.0 | 90.3 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 100 MM MIB PH 5.0, 250 MM AMMONIUM ACETATE, 25% PEG 3350. 4 DEGREES CELSIUS |
