4UEC

Complex of D. melanogaster eIF4E with eIF4G and cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-14
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	54.910, 68.070, 103.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.545 - 2.400
R-factor	0.2119
Rwork	0.209
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4UE8 CHAIN A
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	15790
<I/σ(I)>	15.4	2.3
Completeness [%]	99.9	99.8
Redundancy	6.5	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1M MES PH 6.0, 20% PEG2000 MME