4U4X
Crystal structure of the GluA2 ligand-binding domain (S1S2J-L483Y-N754S) in complex with glutamate and BPAM37 at 1.56 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.290, 121.569, 47.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.036 - 1.560 |
| R-factor | 0.151 |
| Rwork | 0.150 |
| R-free | 0.17490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lb8 molA |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 121.569 | 29.036 | 1.640 |
| High resolution limit [Å] | 1.560 | 4.930 | 1.560 |
| Rmerge | 0.019 | 0.341 | |
| Rmeas | 0.051 | ||
| Rpim | 0.025 | 0.011 | 0.202 |
| Total number of observations | 310291 | 9851 | 44476 |
| Number of reflections | 81474 | ||
| <I/σ(I)> | 14.5 | 31.4 | 3.5 |
| Completeness [%] | 99.8 | 96.5 | 100 |
| Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 280 | 24% PEG4000, 0.1 M ammoniumsulfate, 0.1 M phosphate-citrate |
