4U2R

Crystal structure of the GLUR2 ligand binding core (S1S2J, flip variant) in the apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	66.659, 91.936, 201.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.280 - 1.411
R-factor	0.2131
Rwork	0.212
R-free	0.23170
RMSD bond length	0.011
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	63.280
High resolution limit [Å]	1.410
Number of reflections	404918
<I/σ(I)>	8.68
Completeness [%]	97.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	17-20% PEG8000, 0.2M lithium sulfate, 0.1 M sodium acetate