4U0M
Structure of the Vibrio cholerae di-nucleotide cyclase (DncV) mutant D193N in complex with ATP, GTP and 5MTHFGLU2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.898, 59.860, 104.185 |
| Unit cell angles | 90.00, 95.55, 90.00 |
Refinement procedure
| Resolution | 42.568 - 2.300 |
| R-factor | 0.2015 |
| Rwork | 0.199 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u0l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.704 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 103.700 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 38293 |
| <I/σ(I)> | 12.9 |
| Completeness [%] | 99.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% w/v PEG 3350, 200mM NaCl, 100mM Tris-HCl, 5mM GTP, 5mM ATP |
