4TZC

Crystal Structure of Murine Cereblon in Complex with Thalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-24
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	I 2 3
Unit cell lengths	143.146, 143.146, 143.146
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.880
R-factor	0.1752
Rwork	0.173
R-free	0.21973
RMSD bond length	0.013
RMSD bond angle	1.644
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.880	4.050	1.880
Rmerge	0.089	0.049	0.599
Total number of observations	1750335
Number of reflections	76864
<I/σ(I)>	9.7
Completeness [%]	99.9	100	100
Redundancy	22.8	23.3	23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	100mM Sodium Acetate pH 5, 600-800mM Ammonium Sulfate.