4TZC
Crystal Structure of Murine Cereblon in Complex with Thalidomide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-24 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 143.146, 143.146, 143.146 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.880 |
| R-factor | 0.1752 |
| Rwork | 0.173 |
| R-free | 0.21973 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 4.050 | 1.880 |
| Rmerge | 0.089 | 0.049 | 0.599 |
| Total number of observations | 1750335 | ||
| Number of reflections | 76864 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 99.9 | 100 | 100 |
| Redundancy | 22.8 | 23.3 | 23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 100mM Sodium Acetate pH 5, 600-800mM Ammonium Sulfate. |
