4TZ4

Crystal Structure of Human Cereblon in Complex with DDB1 and Lenalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	71.862, 129.117, 198.677
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.010
R-factor	0.2049
Rwork	0.202
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DDB1
RMSD bond length	0.008
RMSD bond angle	1.211
Data reduction software	HKL-3000
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.120
High resolution limit [Å]	3.010	6.480	3.010
Rmerge	0.181	0.082	0.723
Total number of observations	228575
Number of reflections	37313
<I/σ(I)>	5.9
Completeness [%]	98.5	99.5	85.8
Redundancy	6.1	5.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100mM HEPES pH 7.5, 18% PEG 10K