4TZ4
Crystal Structure of Human Cereblon in Complex with DDB1 and Lenalidomide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.862, 129.117, 198.677 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.010 |
R-factor | 0.2049 |
Rwork | 0.202 |
R-free | 0.27060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DDB1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.211 |
Data reduction software | HKL-3000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.120 |
High resolution limit [Å] | 3.010 | 6.480 | 3.010 |
Rmerge | 0.181 | 0.082 | 0.723 |
Total number of observations | 228575 | ||
Number of reflections | 37313 | ||
<I/σ(I)> | 5.9 | ||
Completeness [%] | 98.5 | 99.5 | 85.8 |
Redundancy | 6.1 | 5.6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM HEPES pH 7.5, 18% PEG 10K |