4TYJ
Structural analysis of the human Fibroblast Growth Factor Receptor 4 Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.281, 66.281, 176.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.030 - 2.450 |
| R-factor | 0.21671 |
| Rwork | 0.214 |
| R-free | 0.24695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | generated using swiss model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.030 | 2.650 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.091 | 0.850 |
| Number of reflections | 15173 | |
| <I/σ(I)> | 0.1734 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.4 M Ammonium dihydrogen phosphate |
