4TY0

Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase in complex with linear intermediate 5' pppA(3',5')pG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-05-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.28180
Spacegroup name	P 1 21 1
Unit cell lengths	70.329, 59.596, 102.163
Unit cell angles	90.00, 96.47, 90.00

Refinement procedure

Resolution	45.345 - 1.800
R-factor	0.1734
Rwork	0.173
R-free	0.20590
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.201
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.345
High resolution limit [Å]	1.800
Number of reflections	76362
<I/σ(I)>	9.7
Completeness [%]	97.2
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	30 mM HEPES-KOH pH 7.5, 200-300 mM Mg(OAc)2 and 20-22% PEG-3350