4TU8

STRUCTURE OF U2AF65 VARIANT WITH BRU5A6 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.920
Spacegroup name	C 1 2 1
Unit cell lengths	164.040, 37.178, 100.710
Unit cell angles	90.00, 125.66, 90.00

Refinement procedure

Resolution	31.068 - 1.918
R-factor	0.1818
Rwork	0.178
R-free	0.21820
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.223
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.070	50.000	1.950
High resolution limit [Å]	1.918	5.210	1.918
Rmerge	0.051	0.039	0.428
Total number of observations	107030
Number of reflections	70516
<I/σ(I)>	11
Completeness [%]	94.3	93	64.3
Redundancy	2.8	1.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	1.6M AMMONIUM SULFATE, 10% DIOXANE, 0.1M MES PH 6.5, ETHYLENE GLYCOL, DEOXY-BIG CHAP