4TQB

The co-complex structure of the translation initiation factor eIF4E with the inhibitor 4EGI-1 reveals an allosteric mechanism for dissociating eIF4G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	CCD
Collection date	2011-07-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.91956
Spacegroup name	P 1 21 1
Unit cell lengths	39.830, 73.480, 66.260
Unit cell angles	90.00, 106.66, 90.00

Refinement procedure

Resolution	19.390 - 1.590
R-factor	0.1832
Rwork	0.182
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.171
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.390
High resolution limit [Å]	1.590
Number of reflections	46347
<I/σ(I)>	14.1
Completeness [%]	94.1
Redundancy	2.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	PEG 4000 12-20%, 100mM MES pH 6.5, 10% IPN