4TN3
Structure of the BBox-Coiled-coil region of Rhesus Trim5alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.118, 59.745, 146.962 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 33.934 - 3.199 |
| R-factor | 0.2609 |
| Rwork | 0.258 |
| R-free | 0.31560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lyd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.685 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.300 |
| High resolution limit [Å] | 3.199 | 3.199 |
| Number of reflections | 17873 | |
| <I/σ(I)> | 11.9 | 2.4 |
| Completeness [%] | 98.9 | 95.6 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% PEG4000, 20% Glycerol, 0.2M monosaccharides, 0.1M Bis/Tris pH 8.5 |
