4TN1

Translation initiation factor eIF5B (517-858) mutant D533R from C. thermophilum, bound to GTPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 41 21 2
Unit cell lengths	115.690, 115.690, 119.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.498 - 2.750
R-factor	0.2083
Rwork	0.206
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ncn
RMSD bond length	0.005
RMSD bond angle	1.031
Data reduction software	XDS
Data scaling software	XSCALE (2.5.2)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.920
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.078	0.016	0.568
Rmeas	0.076	0.018	0.713
Total number of observations	154138
Number of reflections	21704	291	1561
<I/σ(I)>	19.35	74.9	3.1
Completeness [%]	99.8	97	99.1
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	MES, PEG 8000, NaOAc