4TN1
Translation initiation factor eIF5B (517-858) mutant D533R from C. thermophilum, bound to GTPgammaS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 115.690, 115.690, 119.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.498 - 2.750 |
R-factor | 0.2083 |
Rwork | 0.206 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ncn |
RMSD bond length | 0.005 |
RMSD bond angle | 1.031 |
Data reduction software | XDS |
Data scaling software | XSCALE (2.5.2) |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.920 | |
High resolution limit [Å] | 2.750 | 12.300 | 2.750 |
Rmerge | 0.078 | 0.016 | 0.568 |
Rmeas | 0.076 | 0.018 | 0.713 |
Total number of observations | 154138 | ||
Number of reflections | 21704 | 291 | 1561 |
<I/σ(I)> | 19.35 | 74.9 | 3.1 |
Completeness [%] | 99.8 | 97 | 99.1 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | MES, PEG 8000, NaOAc |