4TMX

Translation initiation factor eIF5B (517-858) mutant D533N from C. thermophilum, bound to GTP and sodium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	55.530, 116.820, 66.490
Unit cell angles	90.00, 101.02, 90.00

Refinement procedure

Resolution	46.430 - 1.500
R-factor	0.1564
Rwork	0.155
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ncn
RMSD bond length	0.014
RMSD bond angle	1.619
Data reduction software	XDS
Data scaling software	XSCALE (2.5.2)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.430		1.590
High resolution limit [Å]	1.500	6.710	1.500
Rmerge		0.012
Rmeas	0.029	0.014	0.598
Total number of observations	542891
Number of reflections	131458	1523	9467
<I/σ(I)>	24.4	119.58	2.9
Completeness [%]	99.2	99.5	98.2
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	293	HEPES, PEG 4000, NaOAc