4TMA
Crystal structure of gyrase bound to its inhibitor YacG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.210, 114.460, 462.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.323 - 3.300 |
R-factor | 0.2447 |
Rwork | 0.243 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nuh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.295 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.300 | |
Rmerge | 0.083 | |
Number of reflections | 67289 | |
<I/σ(I)> | 8.1 | |
Completeness [%] | 98.8 | 99.2 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, sodium cacodylate pH 5.0, potassium thiocyanate, spermidine, ZnCl2 |