4S0Z
Crystal structure of M26V human DJ-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.039, 75.039, 75.269 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.990 - 1.450 |
| R-factor | 0.1114 |
| Rwork | 0.110 |
| R-free | 0.13243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5f |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.793 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.062 | 0.523 |
| Number of reflections | 43750 | |
| <I/σ(I)> | 56.6 | 5 |
| Completeness [%] | 99.7 | 97 |
| Redundancy | 20.4 | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 100 mM Tris-HCl pH 9.0, 200 mM sodium acetate trihydrate, 25% PEG 4000 and 3 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
