4RXZ
Crystal Structure of MDMX phosporylated Tyr99 in complex with a 12-mer peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9753 |
Spacegroup name | P 1 |
Unit cell lengths | 28.613, 41.430, 45.618 |
Unit cell angles | 103.32, 103.67, 102.44 |
Refinement procedure
Resolution | 42.330 - 1.550 |
R-factor | 0.18249 |
Rwork | 0.180 |
R-free | 0.22034 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eqy |
RMSD bond length | 0.020 |
RMSD bond angle | 1.964 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.330 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.059 | 0.485 |
Number of reflections | 25745 | |
<I/σ(I)> | 16.14 | 1.1 |
Completeness [%] | 93.0 | 87.4 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% isopropanol, 0.1M MES pH 6.5 and 10% PEG5000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 298K |