4RXC
T. Brucei Farnesyl Diphosphate Synthase Complexed with Homorisedronate BPH-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2008-04-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.012, 120.919, 63.340 |
Unit cell angles | 90.00, 118.33, 90.00 |
Refinement procedure
Resolution | 40.990 - 2.310 |
R-factor | 0.1995 |
Rwork | 0.197 |
R-free | 0.25390 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.684 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Completeness [%] | 91.0 | 56.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 298 | 10% MPD, 0.1 AMMONIUM ACETATE, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 298K |