4RT7

Crystal Structure of FLT3 with a small molecule inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1159
Spacegroup name	P 32 2 1
Unit cell lengths	78.880, 78.880, 138.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.253 - 3.100
R-factor	0.2236
Rwork	0.218
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.898
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	61.253
High resolution limit [Å]	3.100
Number of reflections	8651
Completeness [%]	91.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	100mM Na-Citrate, pH5.5 and 20% PEG3000, VAPOR DIFFUSION, SITTING DROP, temperature 293K