4RSC
Crystal structure of RPE65 in complex with emixustat and palmitate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0750 |
Spacegroup name | P 65 |
Unit cell lengths | 175.891, 175.891, 86.533 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.930 - 1.800 |
R-factor | 0.16623 |
Rwork | 0.165 |
R-free | 0.19569 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fsn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.549 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 139163 |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | 30% PEG200 200 mM ammonium phosphate 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |