4RPP
crystal structure of PKM2-K422R mutant bound with FBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.674, 152.554, 97.676 |
Unit cell angles | 90.00, 104.24, 90.00 |
Refinement procedure
Resolution | 38.313 - 2.585 |
R-factor | 0.2753 |
Rwork | 0.274 |
R-free | 0.30800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.765 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.585 |
Number of reflections | 70651 |
Completeness [%] | 99.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1M Sodium chloride, 0.1M BIS-TRIS propane pH 9.0, 25% PEG1500, VAPOR DIFFUSION, HANGING DROP, temperature 291K |