4RMJ
Human Sirt2 in complex with ADP ribose and nicotinamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.600, 77.960, 114.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.200 - 1.870 |
R-factor | 0.21121 |
Rwork | 0.210 |
R-free | 0.24014 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.595 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.400 | 1.910 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 58048 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.75 | 293 | 18% (wt/vol) PEG 10,000, pH 5.75, VAPOR DIFFUSION, SITTING DROP, temperature 293K |